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[KO Validated] AKT1 Rabbit mAb (A17909)

Publications (23) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] AKT1 Rabbit mAb (A17909)

Western blot analysis of extracts of various cell lines, using AKT1 antibody (A17909) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] AKT1 Rabbit mAb (A17909)

Western blot analysis of extracts from wild type(WT) and AKT1 knockout (KO) HeLa cells, using AKT1 antibody (A17909) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] AKT1 Rabbit mAb (A17909)

Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] AKT1 Rabbit mAb (A17909) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] AKT1 Rabbit mAb (A17909)

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] AKT1 Rabbit mAb (A17909) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] AKT1 Rabbit mAb (A17909)

Immunohistochemistry of paraffin-embedded mouse kidney using [KO Validated] AKT1 Rabbit mAb (A17909) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] AKT1 Rabbit mAb (A17909)

Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] AKT1 Rabbit mAb (A17909) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - [KO Validated] AKT1 Rabbit mAb (A17909)

Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug AKT1 antibody (A17909). Western blot was performed from the immunoprecipitate using AKT1 antibody (A17909) at a dilition of 1:1000.

Overview

Product name[KO Validated] AKT1 Rabbit mAb
Catalog No.A17909
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 381-480 of human AKT1 (P31749).
SequenceSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA
Gene ID207
Swiss protP31749
SynonymsAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT1
Calculated MW56kDa
Observed MW60KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesMCF7, DU145, NIH/3T3, Mouse thymus, Rat heart, Rat testis
Cellular locationCell membrane, Cytoplasm, Nucleus
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

ABclonal:Western blot - [KO Validated] AKT1 Rabbit mAb (A17909)}

Western blot - [KO Validated] AKT1 Rabbit mAb (A17909)

Western blot analysis of extracts of various cell lines, using AKT1 antibody (A17909) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] AKT1 Rabbit mAb (A17909)}

Western blot - [KO Validated] AKT1 Rabbit mAb (A17909)

Western blot analysis of extracts from wild type(WT) and AKT1 knockout (KO) HeLa cells, using AKT1 antibody (A17909) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] AKT1 Rabbit mAb (A17909)}

Immunohistochemistry - [KO Validated] AKT1 Rabbit mAb (A17909)

Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] AKT1 Rabbit mAb (A17909) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] AKT1 Rabbit mAb (A17909)}

Immunohistochemistry - [KO Validated] AKT1 Rabbit mAb (A17909)

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] AKT1 Rabbit mAb (A17909) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] AKT1 Rabbit mAb (A17909)}

Immunohistochemistry - [KO Validated] AKT1 Rabbit mAb (A17909)

Immunohistochemistry of paraffin-embedded mouse kidney using [KO Validated] AKT1 Rabbit mAb (A17909) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] AKT1 Rabbit mAb (A17909)}

Immunohistochemistry - [KO Validated] AKT1 Rabbit mAb (A17909)

Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] AKT1 Rabbit mAb (A17909) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunoprecipitation - [KO Validated] AKT1 Rabbit mAb (A17909)}

Immunoprecipitation - [KO Validated] AKT1 Rabbit mAb (A17909)

Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug AKT1 antibody (A17909). Western blot was performed from the immunoprecipitate using AKT1 antibody (A17909) at a dilition of 1:1000.

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