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[KO Validated] AKT1 Rabbit pAb (A16343)

Publications (4) Datasheet

ABclonal:Western blot - [KO Validated] AKT1 Rabbit pAb (A16343)
ABclonal:Western blot - [KO Validated] AKT1 Rabbit pAb (A16343)
ABclonal:Immunohistochemistry - [KO Validated] AKT1 Rabbit pAb (A16343)
ABclonal:Immunohistochemistry - [KO Validated] AKT1 Rabbit pAb (A16343)

Overview

Product name[KO Validated] AKT1 Rabbit pAb
Catalog No.A16343
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
ImmunogenA synthetic peptide of human AKT1
SequenceEmail for sequence
Gene ID207
Swiss protP31749
SynonymsAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT1
Calculated MW48kDa/55kDa
Observed MW56kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMouse thymus, Rat thymus
Cellular locationCell membrane, Cytoplasm, Nucleus
Customer validation

WB(Mus musculus, Rattus norvegicus, Homo sapiens)

ABclonal:Western blot - [KO Validated] AKT1 Rabbit pAb (A16343) }

Western blot - [KO Validated] AKT1 Rabbit pAb (A16343)

Western blot analysis of extracts of various cell lines, using AKT1 antibody (A16343) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] AKT1 Rabbit pAb (A16343) }

Western blot - [KO Validated] AKT1 Rabbit pAb (A16343)

Western blot analysis of extracts from normal (control) and AKT1 knockout (KO) HeLa cells, using AKT1 antibody (A16343) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Immunohistochemistry - [KO Validated] AKT1 Rabbit pAb (A16343) }

Immunohistochemistry - [KO Validated] AKT1 Rabbit pAb (A16343)

Immunohistochemistry of paraffin-embedded human liver cancer using AKT1 antibody (A16343) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] AKT1 Rabbit pAb (A16343) }

Immunohistochemistry - [KO Validated] AKT1 Rabbit pAb (A16343)

Immunohistochemistry of paraffin-embedded human esophageal using AKT1 antibody (A16343) at dilution of 1:100 (40x lens).

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