Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Kininogen 1 (KNG1) Rabbit pAb (A1670)

Review (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Kininogen 1 (KNG1) Rabbit pAb (A1670)

Western blot analysis of Rat lung, using Kininogen1(KNG1) antibody (A1670) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Kininogen 1 (KNG1) Rabbit pAb (A1670)

Immunofluorescence analysis of HepG2 cells using Kininogen 1 (KNG1) Rabbit pAb (A1670) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for Kininogen 1 (KNG1) Rabbit pAb(A1670)

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Overview

Product name Kininogen 1 (KNG1) Rabbit pAb
Catalog No. A1670
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. Bradykinin also functions as an antimicrobial peptide with antibacterial and antifungal activity. In contrast to HMWK, LMWK is not involved in blood coagulation. Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reduces or depletes angiotensin converting enzyme 2 (ACE2), which results in an increase in levels of des-Arg(9)-bradykinin, a bioactive metabolite of bradykinin that is associated with lung injury and inflammation. Three transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human Kininogen 1 (KNG1) (NP_000884.1).
Sequence PISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRITYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLGMISLMKRPPGFSPFRSSRIGEIKEETTSHLRSCEYKGRPPKAGAEPASEREVS
Gene ID 3827
Swiss prot P01042
Synonyms BK; HK; BDK; KNG; HAE6; HMWK; Kininogen 1 (KNG1)
Calculated MW 72kDa
Observed MW 72kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Rat lung
Cellular location Secreted, extracellular space
Customer validation

WB (Human cells)

Documents

Certificate of Compliance

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Lot number

Research Area

Kininogen 1 (KNG1) Rabbit pAb images

ABclonal:Western blot - Kininogen 1 (KNG1) Rabbit pAb (A1670)}

Western blot - Kininogen 1 (KNG1) Rabbit pAb (A1670)

Western blot analysis of Rat lung, using Kininogen1(KNG1) antibody (A1670) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - Kininogen 1 (KNG1) Rabbit pAb (A1670)}

Immunofluorescence - Kininogen 1 (KNG1) Rabbit pAb (A1670)

Immunofluorescence analysis of HepG2 cells using Kininogen 1 (KNG1) Rabbit pAb (A1670) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on KNG1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to KNG1. (Distance between topics and target gene indicate popularity.) KNG1

* Data provided by citexs.com, for reference only.

Publishing research using A1670? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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