Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

JMJD8 Rabbit pAb (A10476)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - JMJD8 Rabbit pAb (A10476)

Western blot analysis of extracts of various cell lines, using JMJD8 antibody (A10476) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

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Overview

Product name JMJD8 Rabbit pAb
Catalog No. A10476
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Involved in several processes, including positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of sprouting angiogenesis; and regulation of glycolytic process. Located in endoplasmic reticulum lumen and nucleus.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human JMJD8 (NP_001005920.2).
Sequence MAGGWAQGSRRAARLPEGGRLMAPASRLLALWALAAVALPGSGAEGDGGWRPGGPGAVAEEERCTVERRADLTYAEFVQQYAFVRPVILQGLTDNSRFRALCSRDRLLASFGDRVVRLSTANTYSYHKVDLPFQEYVEQLLHPQDPTSLGNDTLYFFGDNNFTEWASLFRHYSPPPFGLLGTAPAYSFGIAGAGSGVPFHWHGPGYSEVIYGRKRWFLYPPEKTPEFHPNKTTLAWLRDTYPALPPSARPLECTIRAGEVLYFPDRWWHATLNLDTSVFISTFLG
Gene ID 339123
Swiss prot Q96S16
Synonyms PP14397; C16orf20; JMJD8
Calculated MW 30kDa
Observed MW 37kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T, HeLa, HL-60, Jurkat, Mouse kidney, Mouse liver, Rat testis, Rat liver
Cellular location cytoplasm, endoplasmic reticulum, endoplasmic reticulum lumen, nucleus

Documents

Certificate of Compliance

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JMJD8 Rabbit pAb images

ABclonal:Western blot - JMJD8 Rabbit pAb (A10476)}

Western blot - JMJD8 Rabbit pAb (A10476)

Western blot analysis of extracts of various cell lines, using JMJD8 antibody (A10476) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

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Related Targets

Based on existing publications, the following genes are closely related to research on JMJD8. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to JMJD8. (Distance between topics and target gene indicate popularity.) JMJD8

* Data provided by citexs.com, for reference only.

Publishing research using A10476? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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