Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Integrin-β1/CD29 Rabbit mAb (A11060)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Integrin-β1/CD29 Rabbit mAb (A11060)

Western blot analysis of various lysates using Integrin-β1/CD29 Rabbit mAb (A11060) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Overview

Product name Integrin-β1/CD29 Rabbit mAb
Catalog No. A11060
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant protein of human ITGB1
Sequence Email for sequence
Gene ID 3688
Swiss prot P05556
Synonyms CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA; Integrin-β1/CD29
Calculated MW 88kDa
Observed MW 100-130kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HepG2, HeLa, U-87MG, HT-1080, Mouse kidney
Cellular location Cell junction, Cell membrane, Cell projection, Cleavage furrow, Melanosome, Recycling endosome, Single-pass type I membrane protein, invadopodium membrane, lamellipodium, ruffle membrane, ruffle, sarcolemma
Customer validation

WB (Rattus norvegicus, Homo sapiens)

IF (Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

Integrin-β1/CD29 Rabbit mAb images

ABclonal:Western blot - Integrin-β1/CD29 Rabbit mAb (A11060)}

Western blot - Integrin-β1/CD29 Rabbit mAb (A11060)

Western blot analysis of various lysates using Integrin-β1/CD29 Rabbit mAb (A11060) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ITGB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ITGB1. (Distance between topics and target gene indicate popularity.) ITGB1

* Data provided by citexs.com, for reference only.

Publishing research using A11060? Please let us know so that we can cite the reference in this datasheet.

Antibodies (8)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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