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Integrin-β1/CD29 Rabbit mAb (A11060)

Publications (2) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Integrin-β1/CD29 Rabbit mAb (A11060)

Western blot analysis of extracts of various cell lines, using ITGB1 antibody (A11060) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameIntegrin-β1/CD29 Rabbit mAb
Catalog No.A11060
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
ImmunogenRecombinant protein of human ITGB1
SequenceEmail for sequence
Gene ID3688
Swiss protP05556
SynonymsCD29; FNRB; GPIIA; MDF2; MSK12; VLA-BETA; VLAB; Integrin beta 1; ITGB1; Integrin β1
Calculated MW87kDa/88kDa/91kDa
Observed MW100-130kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHepG2, HeLa, U-87MG, HT-1080, Mouse kidney
Cellular locationCell junction, Cell membrane, Cell projection, Cleavage furrow, Melanosome, Recycling endosome, Single-pass type I membrane protein, invadopodium membrane, lamellipodium, ruffle membrane, ruffle, sarcolemma
Customer validation

WB(Homo sapiens, Rattus norvegicus)

IF(Rattus norvegicus)

ABclonal:Western blot - Integrin-β1/CD29 Rabbit mAb (A11060) }

Western blot - Integrin-β1/CD29 Rabbit mAb (A11060)

Western blot analysis of extracts of various cell lines, using ITGB1 antibody (A11060) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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