Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Integrin-β1/CD29 Rabbit pAb (A2217)

Publications (9) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Integrin-β1/CD29 Rabbit pAb (A2217)

Western blot analysis of extracts of various cell lines, using Integrin-β1/CD29 antibody (A2217) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - Integrin-β1/CD29 Rabbit pAb (A2217)

Immunofluorescence analysis of NIH-3T3 cells using Integrin-β1/CD29 Rabbit pAb (A2217) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name Integrin-β1/CD29 Rabbit pAb
Catalog No. A2217
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-728 of human Integrin-β1/CD29 (NP_002202.2).
Sequence MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPD
Gene ID 3688
Swiss prot P05556
Synonyms CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA; Integrin-β1/CD29
Calculated MW 88kDa
Observed MW 135kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples A-431, U-87MG
Cellular location Cell junction, Cell membrane, Cell projection, Cleavage furrow, Melanosome, Recycling endosome, Single-pass type I membrane protein, invadopodium membrane, lamellipodium, ruffle membrane, ruffle, sarcolemma
Customer validation

WB (Homo sapiens, Capra hircus, Rattus norvegicus, Mus musculus)

IF (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Integrin-β1/CD29 Rabbit pAb images

ABclonal:Western blot - Integrin-β1/CD29 Rabbit pAb (A2217)}

Western blot - Integrin-β1/CD29 Rabbit pAb (A2217)

Western blot analysis of extracts of various cell lines, using Integrin-β1/CD29 antibody (A2217) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - Integrin-β1/CD29 Rabbit pAb (A2217)}

Immunofluorescence - Integrin-β1/CD29 Rabbit pAb (A2217)

Immunofluorescence analysis of NIH-3T3 cells using Integrin-β1/CD29 Rabbit pAb (A2217) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on ITGB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ITGB1. (Distance between topics and target gene indicate popularity.) ITGB1

* Data provided by citexs.com, for reference only.

Publishing research using A2217? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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