Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

IRS1 Rabbit pAb (A0245)

Review (1) Publications (10) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - IRS1 Rabbit pAb (A0245)

Western blot analysis of extracts of various cell lines, using IRS1 antibody (A0245) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - IRS1 Rabbit pAb (A0245)

Western blot analysis of extracts of 293T cells, using IRS1 antibody (A0245) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - IRS1 Rabbit pAb (A0245)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - IRS1 Rabbit pAb (A0245)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - IRS1 Rabbit pAb (A0245)

Immunohistochemistry analysis of paraffin-embedded rat brain using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - IRS1 Rabbit pAb (A0245)

Immunofluorescence analysis of A-549 cells using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IRS1 Rabbit pAb (A0245)

Immunofluorescence analysis of MCF7 cells using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IRS1 Rabbit pAb (A0245)

Immunofluorescence analysis of NIH/3T3 cells using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IRS1 Rabbit pAb (A0245)

Immunofluorescence analysis of PC-12 cells using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for IRS1 Rabbit pAb(A0245)

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Overview

Product name IRS1 Rabbit pAb
Catalog No. A0245
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1043-1242 of human IRS1 (NP_005535.1).
Sequence SPTGPQGAAELAAHSSLLGGPQGPGGMSAFTRVNLSPNRNQSAKVIRADPQGCRRRHSSETFSSTPSATRVGNTVPFGAGAAVGGGGGSSSSSEDVKRHSSASFENVWLRPGELGGAPKEPAKLCGAAGGLENGLNYIDLDLVKDFKQCPQECTPEPQPPPPPPPHQPLGSGESSSTRRSSEDLSAYASISFQKQPEDRQ
Gene ID 3667
Swiss prot P35568
Synonyms HIRS-1; IRS1
Calculated MW 132kDa
Observed MW 180kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples 293T, Mouse liver, Rat liver
Cellular location caveola, cytoplasm, cytosol, nucleoplasm, nucleus, plasma membrane
Customer validation

WB (mouse stomach cellsHomo sapiens, Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

IRS1 Rabbit pAb images

ABclonal:Western blot - IRS1 Rabbit pAb (A0245)}

Western blot - IRS1 Rabbit pAb (A0245)

Western blot analysis of extracts of various cell lines, using IRS1 antibody (A0245) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - IRS1 Rabbit pAb (A0245)}

Western blot - IRS1 Rabbit pAb (A0245)

Western blot analysis of extracts of 293T cells, using IRS1 antibody (A0245) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - IRS1 Rabbit pAb (A0245)}

Immunohistochemistry - IRS1 Rabbit pAb (A0245)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - IRS1 Rabbit pAb (A0245)}

Immunohistochemistry - IRS1 Rabbit pAb (A0245)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - IRS1 Rabbit pAb (A0245)}

Immunohistochemistry - IRS1 Rabbit pAb (A0245)

Immunohistochemistry analysis of paraffin-embedded rat brain using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - IRS1 Rabbit pAb (A0245)}

Immunofluorescence - IRS1 Rabbit pAb (A0245)

Immunofluorescence analysis of A-549 cells using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IRS1 Rabbit pAb (A0245)}

Immunofluorescence - IRS1 Rabbit pAb (A0245)

Immunofluorescence analysis of MCF7 cells using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IRS1 Rabbit pAb (A0245)}

Immunofluorescence - IRS1 Rabbit pAb (A0245)

Immunofluorescence analysis of NIH/3T3 cells using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IRS1 Rabbit pAb (A0245)}

Immunofluorescence - IRS1 Rabbit pAb (A0245)

Immunofluorescence analysis of PC-12 cells using IRS1 Rabbit pAb (A0245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on IRS1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to IRS1. (Distance between topics and target gene indicate popularity.) IRS1

* Data provided by citexs.com, for reference only.

Publishing research using A0245? Please let us know so that we can cite the reference in this datasheet.

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