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IRF7 Rabbit pAb (A0159)

Publication (1) Datasheet

ABclonal:Western blot - IRF7 Polyclonal Antibody (A0159)
ABclonal:Immunofluorescence - IRF7 Polyclonal Antibody (A0159)

Overview

Product nameIRF7 Rabbit pAb
Catalog No.A0159
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 257-516 of human IRF7 (NP_004022.2).
SequencePSPGPQPAALTTGEAAAPESPHQAEPYLSPSPSACTAVQEPSPGALDVTIMYKGRTVLQKVVGHPSCTFLYGPPDPAVRATDPQQVAFPSPAELPDQKQLRYTEELLRHVAPGLHLELRGPQLWARRMGKCKVYWEVGGPPGSASPSTPACLLPRNCDTPIFDFRVFFQELVEFRARQRRGSPRYTIYLGFGQDLSAGRPKEKSLVLVKLEPWLCRVHLEGTQREGVSSLDSSSLSLCLSSANSLYDDIECFLMELEQPA
Gene ID3665
Swiss protQ92985
SynonymsIRF7; IMD39; IRF-7H; IRF7A; IRF7B; IRF7C; IRF7H
Calculated MW18kDa/51kDa/54kDa/55kDa
Observed MW70kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesJurkat, 293T
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Cynoglossus semilaevis)

ABclonal:Western blot - IRF7 Polyclonal Antibody (A0159) }

Western blot - IRF7 Polyclonal Antibody (A0159)

Western blot analysis of extracts of various cell lines, using IRF7 antibody (A0159) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunofluorescence - IRF7 Polyclonal Antibody (A0159) }

Immunofluorescence - IRF7 Polyclonal Antibody (A0159)

Immunofluorescence analysis of U2OS cells using IRF7 antibody (A0159) at dilution of 1:100. Blue: DAPI for nuclear staining.

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