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IRF5 Rabbit pAb (A1149)

Publications (2) Datasheet

ABclonal:Western blot - IRF5 Polyclonal Antibody (A1149)

Western blot analysis of extracts of various cell lines, using IRF5 antibody (A1149) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunofluorescence - IRF5 Polyclonal Antibody (A1149)

Immunofluorescence analysis of HeLa cells using IRF5 antibody (A1149). Blue: DAPI for nuclear staining.

Overview

Product nameIRF5 Rabbit pAb
Catalog No.A1149
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human IRF5 (NP_001092097.2).
SequenceMNQSIPVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHATRHGPSQDGDNTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFRLIYDGPRDMPPQPYKIYEVCSNGPAPTDSQPPEDYSFGAGEEEEEEEELQRMLPSLSLTEDVKWPPTLQPPTLRPPTLQPPTLQPPVVLGPPAPDPSPL
Gene ID3663
Swiss protQ13568
SynonymsIRF5; SLEB10
Calculated MW17kDa/47kDa/54kDa/56kDa/57kDa
Observed MW56kDa/60kDa
ReactivityHuman, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesTHP-1, U-937, Rat thymus
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens,Mus musculus)

IHC(Rattus norvegicus)

ABclonal:Western blot - IRF5 Polyclonal Antibody (A1149) }

Western blot - IRF5 Polyclonal Antibody (A1149)

Western blot analysis of extracts of various cell lines, using IRF5 antibody (A1149) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunofluorescence - IRF5 Polyclonal Antibody (A1149) }

Immunofluorescence - IRF5 Polyclonal Antibody (A1149)

Immunofluorescence analysis of HeLa cells using IRF5 antibody (A1149). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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