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IQWD1 Polyclonal Antibody (A14286)

Datasheet

ABclonal:Western blot - IQWD1 Polyclonal Antibody (A14286)

Overview

Product nameIQWD1 Polyclonal Antibody
Catalog No.A14286
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a ligand-dependent coactivator of nuclear receptors, including nuclear receptor subfamily 3 group C member 1 (NR3C1), glucocorticoid receptor (GR), and androgen receptor (AR). The encoded protein and DNA damage binding protein 2 (DDB2) may act as tumor promoters and tumor suppressors, respectively, by regulating the level of androgen receptor in prostate tissues. In addition, this protein can act with glucocorticoid receptor to promote human papillomavirus gene expression.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 460-650 of human IQWD1 (NP_001017977.1).
SequenceDNNNEKLSPKPGTGEPVLSLHYSTEGTTTSTIKLNFTDEWSSIASSSRGIGSHCKSEGQEESFVPQSSVQPPEGDSETKAPEESSEDVTKYQEGVSAENPVENHINITQSDKFTAKPLDSNSGERNDLNLDRSCGVPEESASSEKAKEPETSDQTSTESATNENNTNPEPQFQTEATGPSAHEETSTRDSA
Gene ID55827
Swiss protQ58WW2
SynonymsDCAF6; 1200006M05Rik; ARCAP; IQWD1; MSTP055; NRIP; PC326; DDB1 and CUL4 associated factor 6
Calculated MW96kDa/98kDa/103kDa/106kDa
Observed MW105kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, LO2
Cellular locationNucleus
ABclonal:Western blot - IQWD1 Polyclonal Antibody (A14286) }

Western blot - IQWD1 Polyclonal Antibody (A14286)

Western blot analysis of extracts of various cell lines, using IQWD1 antibody (A14286) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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