Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

IL5 Rabbit pAb (A7690)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - IL5 Rabbit pAb (A7690)

Western blot analysis of various lysates using IL5 Rabbit pAb (A7690) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 15s.

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Overview

Product name IL5 Rabbit pAb
Catalog No. A7690
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 20-134 of human IL5 (NP_000870.1).
Sequence IPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES
Gene ID 3567
Swiss prot P05113
Synonyms EDF; TRF; IL-5; IL5
Calculated MW 15kDa
Observed MW 13kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse thymus, Mouse liver, Rat thymus
Cellular location Secreted
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

IL5 Rabbit pAb images

ABclonal:Western blot - IL5 Rabbit pAb (A7690)}

Western blot - IL5 Rabbit pAb (A7690)

Western blot analysis of various lysates using IL5 Rabbit pAb (A7690) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 15s.

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Related Targets

Based on existing publications, the following genes are closely related to research on IL5. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to IL5. (Distance between topics and target gene indicate popularity.) IL5

* Data provided by citexs.com, for reference only.

Publishing research using A7690? Please let us know so that we can cite the reference in this datasheet.

ELISA Kits (2)

Proteins (1)

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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