Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

IL17RA Rabbit pAb (A10052)

Publications (6) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - IL17RA Rabbit pAb (A10052)

Western blot analysis of extracts of various cell lines, using IL17RA antibody (A10052) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Overview

Product name IL17RA Rabbit pAb
Catalog No. A10052
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The transmembrane protein encoded by this gene (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 33-320 of human IL17RA (NP_055154.3).
Sequence LRLLDHRALVCSQPGLNCTVKNSTCLDDSWIHPRNLTPSSPKDLQIQLHFAHTQQGDLFPVAHIEWTLQTDASILYLEGAELSVLQLNTNERLCVRFEFLSKLRHHHRRWRFTFSHFVVDPDQEYEVTVHHLPKPIPDGDPNHQSKNFLVPDCEHARMKVTTPCMSSGSLWDPNITVETLEAHQLRVSFTLWNESTHYQILLTSFPHMENHSCFEHMHHIPAPRPEEFHQRSNVTLTLRNLKGCCRHQVQIQPFFSSCLNDCLRHSATVSCPEMPDTPEPIPDYMPLW
Gene ID 23765
Swiss prot Q96F46
Synonyms CD217; IL17R; IMD51; CANDF5; CDw217; IL-17RA; hIL-17R; IL17RA
Calculated MW 96kDa
Observed MW 100kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:4000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HL-60, Mouse liver, Mouse kidney, Mouse lung, Mouse thymus, Rat liver
Cellular location Membrane, Secreted, Single-pass type I membrane protein
Customer validation

WB (Rattus norvegicus, Homo sapiens, Mus musculus)

qRT-QCR (Mus musculus)

FCM (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

IL17RA Rabbit pAb images

ABclonal:Western blot - IL17RA Rabbit pAb (A10052)}

Western blot - IL17RA Rabbit pAb (A10052)

Western blot analysis of extracts of various cell lines, using IL17RA antibody (A10052) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Related Targets

Based on existing publications, the following genes are closely related to research on IL17RA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to IL17RA. (Distance between topics and target gene indicate popularity.) IL17RA

* Data provided by citexs.com, for reference only.

Publishing research using A10052? Please let us know so that we can cite the reference in this datasheet.

Proteins (3)

Antibodies (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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