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IL10 Rabbit pAb (A2171)

Review (1)Publications (11) Datasheet

ABclonal:Western blot - IL10 Polyclonal Antibody (A2171)

Western blot analysis of extracts of Jurkat cells, using IL10 antibody (A2171) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

ABclonal:Immunohistochemistry - IL10 Polyclonal Antibody (A2171)

Immunohistochemistry of paraffin-embedded mouse spleen using IL10 antibody (A2171) at dilution of 1:200 (40x lens).

Review
ABclonal: review for IL10 Rabbit pAb(A2171)

Overview

Product nameIL10 Rabbit pAb
Catalog No.A2171
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 19-178 of human IL10 (NP_000563.1).
SequenceSPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN
Gene ID3586
Swiss protP22301
SynonymsCSIF; GVHDS; IL-10; IL10A; TGIF; IL10
Calculated MW20kDa
Observed MW22kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesJurkat
Cellular locationSecreted
Customer validation

(Mouse embryonal fibroblast cell)

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Cynoglossus semilaevis)

IF(Homo sapiens)

ABclonal:Western blot - IL10 Polyclonal Antibody (A2171) }

Western blot - IL10 Polyclonal Antibody (A2171)

Western blot analysis of extracts of Jurkat cells, using IL10 antibody (A2171) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.
ABclonal:Immunohistochemistry - IL10 Polyclonal Antibody (A2171) }

Immunohistochemistry - IL10 Polyclonal Antibody (A2171)

Immunohistochemistry of paraffin-embedded mouse spleen using IL10 antibody (A2171) at dilution of 1:200 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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