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ICAM1 Rabbit pAb (A5597)

Publication (4) Datasheet

ABclonal:Western blot - ICAM1 Polyclonal Antibody (A5597)
ABclonal:Immunofluorescence - ICAM1 Polyclonal Antibody (A5597)

Overview

Product nameICAM1 Rabbit pAb
Catalog No.A5597
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 250-480 of human ICAM1 (NP_000192.2).
SequenceVHLALGDQRLNPTVTYGNDSFSAKASVSVTAEDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNVILTKPEVSEGTEVTVKCEAHPRAKVTLNGVPAQPLGPRAQLLLKATPEDNGRSFSCSATLEVAGQLIHKNQTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQAWGNPLPELKCLKDGTFPLPIGESVTVTRDLEGTYLCRARSTQGEVTRKVTVNVLSPRYE
Gene ID3383
Swiss protP05362
SynonymsBB2; CD54; P3.58; ICAM1
Calculated MW57kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesRaji, Mouse lung, Rat lung
Cellular locationMembrane, Single-pass type I membrane protein
Customer validation

WB(Mouse, Rat)

IF(Pig)

ABclonal:Western blot - ICAM1 Polyclonal Antibody (A5597) }

Western blot - ICAM1 Polyclonal Antibody (A5597)

Western blot analysis of extracts of various cell lines, using ICAM1 antibody (A5597) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunofluorescence - ICAM1 Polyclonal Antibody (A5597) }

Immunofluorescence - ICAM1 Polyclonal Antibody (A5597)

Immunofluorescence analysis of PC12 cells using ICAM1 antibody (A5597) at dilution of 1:100. Blue: DAPI for nuclear staining.

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