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HSPB1 Polyclonal Antibody (A0240)

Reviews (4)Publications (4) Datasheet

ABclonal:Western blot - HSPB1 Polyclonal Antibody (A0240)
ABclonal:Immunofluorescence - HSPB1 Polyclonal Antibody (A0240)
ABclonal:Immunofluorescence - HSPB1 Polyclonal Antibody (A0240)
ABclonal:Immunoprecipitation - HSPB1 Polyclonal Antibody (A0240)
Review
ABclonal: review for HSPB1 Polyclonal Antibody(A0240)
Review
ABclonal: review for HSPB1 Polyclonal Antibody(A0240)
Review
ABclonal: review for HSPB1 Polyclonal Antibody(A0240)
Review
ABclonal: review for HSPB1 Polyclonal Antibody(A0240)

Overview

Product nameHSPB1 Polyclonal Antibody
Catalog No.A0240
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSPB1 (NP_001531.1).
SequenceMTERRVPFSLLRGPSWDPFRDWYPHSRLFDQAFGLPRLPEEWSQWLGGSSWPGYVRPLPPAAIESPAVAAPAYSRALSRQLSSGVSEIRHTADRWRVSLDVNHFAPDELTVKTKDGVVEI
Gene ID3315
Swiss protP04792
SynonymsHSPB1; CMT2F; HEL-S-102; HMN2B; HS.76067; HSP27; HSP28; Hsp25; SRP27; heat shock protein beta-1
Calculated MW22kDa
Observed MW25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHepG2, HeLa
Cellular locationCytoplasm, Nucleus, cytoskeleton, spindle
Customer validation

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WB(Human, Rat, T. gondii)

ABclonal:Western blot - HSPB1 Polyclonal Antibody (A0240) }

Western blot - HSPB1 Polyclonal Antibody (A0240)

Western blot analysis of extracts of various cell lines, using HSPB1 antibody (A0240) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - HSPB1 Polyclonal Antibody (A0240) }

Immunofluorescence - HSPB1 Polyclonal Antibody (A0240)

Immunofluorescence analysis of C6 cells using HSPB1 antibody (A0240) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HSPB1 Polyclonal Antibody (A0240) }

Immunofluorescence - HSPB1 Polyclonal Antibody (A0240)

Immunofluorescence analysis of HeLa cells using HSPB1 antibody (A0240) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - HSPB1 Polyclonal Antibody (A0240) }

Immunoprecipitation - HSPB1 Polyclonal Antibody (A0240)

Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug HSPB1 antibody (A0240). Western blot was performed from the immunoprecipitate using HSPB1 antibody (A0240) at a dilition of 1:1000.

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