Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Hsp90α Rabbit pAb (A0365)

Reviews (4) Publications (8) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Hsp90α Rabbit pAb (A0365)

Western blot analysis of various lysates using Hsp90α Rabbit pAb (A0365) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - Hsp90α Rabbit pAb (A0365)

Immunofluorescence analysis of U2OS cells using Hsp90α Rabbit pAb (A0365).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Review
ABclonal: review for Hsp90α Rabbit pAb(A0365)
Review
ABclonal: review for Hsp90α Rabbit pAb(A0365)
Review
ABclonal: review for Hsp90α Rabbit pAb(A0365)
Review
ABclonal: review for Hsp90α Rabbit pAb(A0365)

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Overview

Product name Hsp90α Rabbit pAb
Catalog No. A0365
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 433-732 of human Hsp90α (NP_005339.3).
Sequence NYKKFYEQFSKNIKLGIHEDSQNRKKLSELLRYYTSASGDEMVSLKDYCTRMKENQKHIYYITGETKDQVANSAFVERLRKHGLEVIYMIEPIDEYCVQQLKEFEGKTLVSVTKEGLELPEDEEEKKKQEEKKTKFENLCKIMKDILEKKVEKVVVSNRLVTSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMAAKKHLEINPDHSIIETLRQKAEADKNDKSVKDLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGIDEDDPTADDTSAAVTEEMPPLEGDDDTSRMEEVD
Gene ID 3320
Swiss prot P07900
Synonyms EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p; Hsp90α
Calculated MW 85kDa
Observed MW 90kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples U-251MG, HeLa, NIH/3T3, Rat brain, PC-12
Cellular location Cell membrane, Cytoplasm, Melanosome
Customer validation

IHC (Mouse Embryonic Stem Cells)

WB (Human liver cell, 293THomo sapiens, Homo sapiens, Mus musculus)

IF (Human liver cellMus musculus, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Hsp90α Rabbit pAb images

ABclonal:Western blot - Hsp90α Rabbit pAb (A0365)}

Western blot - Hsp90α Rabbit pAb (A0365)

Western blot analysis of various lysates using Hsp90α Rabbit pAb (A0365) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunofluorescence - Hsp90α Rabbit pAb (A0365)}

Immunofluorescence - Hsp90α Rabbit pAb (A0365)

Immunofluorescence analysis of U2OS cells using Hsp90α Rabbit pAb (A0365).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on HSP90AA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HSP90AA1. (Distance between topics and target gene indicate popularity.) HSP90AA1

* Data provided by citexs.com, for reference only.

Publishing research using A0365? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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