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HSD17B2 Polyclonal Antibody (A1983)

Datasheet

ABclonal:Western blot - HSD17B2 Polyclonal Antibody (A1983)

Overview

Product nameHSD17B2 Polyclonal Antibody
Catalog No.A1983
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 118-387 of human HSD17B2 (NP_002144.1).
SequenceGPGAEELRRTCSPRLSVLQMDITKPVQIKDAYSKVAAMLQDRGLWAVINNAGVLGFPTDGELLLMTDYKQCMAVNFFGTVEVTKTFLPLLRKSKGRLVNVSSMGGGAPMERLASYGSSKAAVTMFSSVMRLELSKWGIKVASIQPGGFLTNIAGTSDKWEKLEKDILDHLPAEVQEDYGQDYILAQRNFLLLINSLASKDFSPVLRDIQHAILAKSPFAYYTPGKGAYLWICLAHYLPIGIYDYFAKRHFGQDKPMPRALRMPNYKKKAT
Gene ID3294
Swiss protP37059
SynonymsHSD17B2; EDH17B2; HSD17; SDR9C2; estradiol 17-beta-dehydrogenase 2
Calculated MW42kDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesSW480, Mouse liver, Mouse kidney, Mouse craniofacial, Mouse intestine
Cellular locationMembrane, Single-pass type II membrane protein
ABclonal:Western blot - HSD17B2 Polyclonal Antibody (A1983) }

Western blot - HSD17B2 Polyclonal Antibody (A1983)

Western blot analysis of extracts of various cell lines, using HSD17B2 antibody (A1983) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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