Product Type > Antibodies > Tag and Loading Control Antibodies

HRP-conjugated Mouse anti His-Tag mAb (AE028)

Publications (20) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Species independent

ABclonal:Western blot - HRP-conjugated Mouse anti His-Tag mAb (AE028)

Western blot analysis of extracts of normal 293T cells and 293T transfected with His-NAA20 protein, using HRP-conjugated Mouse anti His-Tag mAb (AE028) at 1:20000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name HRP-conjugated Mouse anti His-Tag mAb
Catalog No. AE028
Host species Mouse
Purification method Affinity purification
Isotype IgG2B, Kappa
CloneNo. AMC0484

Background

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.

Immunogen information

Immunogen A synthetic peptide corresponding to His tag.
Sequence HHHHHH
Gene ID
Swiss prot
Synonyms His; His tag; His-tag
Calculated MW
Observed MW Refer to Figures

Applications

Reactivity Species independent
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:10000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T
Cellular location
Customer validation

WB (Arabidopsis thaliana, Homo sapiens, Oryza sativa, Mus musculus, Danio rerio, Triticum aestivum, Listeria monocytogenes, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Glycine max, Other)

ELISA (Vibrio harveyi , Bacillus cereus)

Documents

Certificate of Compliance

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Lot number

HRP-conjugated Mouse anti His-Tag mAb images

ABclonal:Western blot - HRP-conjugated Mouse anti His-Tag mAb (AE028)}

Western blot - HRP-conjugated Mouse anti His-Tag mAb (AE028)

Western blot analysis of extracts of normal 293T cells and 293T transfected with His-NAA20 protein, using HRP-conjugated Mouse anti His-Tag mAb (AE028) at 1:20000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on His. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to His. (Distance between topics and target gene indicate popularity.) His

* Data provided by citexs.com, for reference only.

Publishing research using AE028? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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