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Heparanase 1 Polyclonal Antibody (A16488)

Publication (1) Datasheet

ABclonal:Western blot - Heparanase 1 Polyclonal Antibody (A16488)
ABclonal:Immunofluorescence - Heparanase 1 Polyclonal Antibody (A16488)
ABclonal:Immunofluorescence - Heparanase 1 Polyclonal Antibody (A16488)

Overview

Product nameHeparanase 1 Polyclonal Antibody
Catalog No.A16488
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant protein of human Heparanase 1
SequenceEmail for sequence
Gene ID10855
Swiss protQ9Y251
SynonymsHPSE; HPA; HPA1; HPR1; HPSE1; HSE1; heparanase
Calculated MW42kDa/53kDa/54kDa/61kDa
Observed MW50kDa/65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, BxPC-3, A-431, mouse spleen, mouse lung, rat spleen, rat lung
Cellular locationLysosome membrane, Nucleus, Peripheral membrane protein, Secreted
Customer validation

WB(Mouse)

IHC(Mouse)

ABclonal:Western blot - Heparanase 1 Polyclonal Antibody (A16488) }

Western blot - Heparanase 1 Polyclonal Antibody (A16488)

Western blot analysis of extracts of various cells, using Heparanase 1 antibody (A16488) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - Heparanase 1 Polyclonal Antibody (A16488) }

Immunofluorescence - Heparanase 1 Polyclonal Antibody (A16488)

Immunofluorescence analysis of HeLa cells using Heparanase 1 antibody (A16488) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Heparanase 1 Polyclonal Antibody (A16488) }

Immunofluorescence - Heparanase 1 Polyclonal Antibody (A16488)

Immunofluorescence analysis of NIH/3T3 cells using Heparanase 1 antibody (A16488) at dilution of 1:100. Blue: DAPI for nuclear staining.

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