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HNF4A Polyclonal Antibody (A2085)

Publication (1) Datasheet

ABclonal:Western blot - HNF4A Polyclonal Antibody (A2085)
ABclonal:Immunohistochemistry - HNF4A Polyclonal Antibody (A2085)
ABclonal:Immunohistochemistry - HNF4A Polyclonal Antibody (A2085)

Overview

Product nameHNF4A Polyclonal Antibody
Catalog No.A2085
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human HNF4A (NP_000448.3).
SequenceEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAK
Gene ID3172
Swiss protP41235
SynonymsHNF4A; FRTS4; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14
Calculated MW43-56kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHepG2, 293T, SW620, Mouse liver, Mouse kidney, Mouse pancreas, Rat liver, Rat kidney
Cellular locationNucleus
Customer validation

WB(Human)

ABclonal:Western blot - HNF4A Polyclonal Antibody (A2085) }

Western blot - HNF4A Polyclonal Antibody (A2085)

Western blot analysis of extracts of various cell lines, using HNF4A antibody (A2085) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - HNF4A Polyclonal Antibody (A2085) }

Immunohistochemistry - HNF4A Polyclonal Antibody (A2085)

Immunohistochemistry of paraffin-embedded mouse liver using HNF4A Antibody (A2085) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - HNF4A Polyclonal Antibody (A2085) }

Immunohistochemistry - HNF4A Polyclonal Antibody (A2085)

Immunohistochemistry of paraffin-embedded rat pancreas using HNF4A Antibody (A2085) at dilution of 1:100 (40x lens).

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