Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

HMGCR Rabbit mAb (A19063)

Publications (4) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - HMGCR Rabbit mAb (A19063)

Western blot analysis of various lysates using HMGCR Rabbit mAb (A19063) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name HMGCR Rabbit mAb
Catalog No. A19063
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0496

Background

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 400-500 of human HMGCR (P04035).
Sequence IKPLVAETDTPNRATFVVGNSSLLDTSSVLVTQEPEIELPREPRPNEECLQILGNAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLS
Gene ID 3156
Swiss prot P04035
Synonyms LDLCQ3; HMGCR
Calculated MW 97kDa
Observed MW 100kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
WB HumanMouseRat
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T, Jurkat, U-87 MG, Mouse brain, Rat liver, Rat brain
Cellular location Endoplasmic reticulum membrane, Multi-pass membrane protein
Customer validation

WB (Rattus norvegicus, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

HMGCR Rabbit mAb images

ABclonal:Western blot - HMGCR Rabbit mAb (A19063)}

Western blot - HMGCR Rabbit mAb (A19063)

Western blot analysis of various lysates using HMGCR Rabbit mAb (A19063) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on HMGCR. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HMGCR. (Distance between topics and target gene indicate popularity.) HMGCR

* Data provided by citexs.com, for reference only.

Publishing research using A19063? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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