Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Histone H3 Rabbit pAb (A2348)

Reviews (5) Publications (159) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Histone H3 Rabbit pAb (A2348)

Western blot analysis of various lysates using Histone H3 Rabbit pAb (A2348) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

Immunohistochemistry analysis of Histone H3 in paraffin-embedded rat lung using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

Immunohistochemistry analysis of Histone H3 in paraffin-embedded human liver using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

Immunohistochemistry analysis of Histone H3 in paraffin-embedded human brain using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

Immunohistochemistry analysis of Histone H3 in paraffin-embedded mouse kidney using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Histone H3 Rabbit pAb (A2348)

Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse testis using Histone H3 Rabbit pAb (A2348) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Histone H3 Rabbit pAb (A2348)

Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat testis using Histone H3 Rabbit pAb (A2348) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Histone H3 Rabbit pAb (A2348)

Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 10ug Histone H3 antibody (A2348). Western blot was performed from the immunoprecipitate using Histone H3 antibody (A2348) at a dilution of 1:30000.

ABclonal:Immunoprecipitation - Histone H3 Rabbit pAb (A2348)

Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 10ug Histone H3 antibody (A2348). Western blot was performed from the immunoprecipitate using Histone H3 antibody (A2348) at a dilution of 1:30000.

ABclonal:Chromatin Immunoprecipitation - Histone H3 Rabbit pAb (A2348)

Chromatin immunoprecipitation analysis of extracts of 293T cells, using Histone H3 antibody (A2348) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review
ABclonal: review for Histone H3 Rabbit pAb(A2348)
Review
ABclonal: review for Histone H3 Rabbit pAb(A2348)
Review
ABclonal: review for Histone H3 Rabbit pAb(A2348)
Review
ABclonal: review for Histone H3 Rabbit pAb(A2348)
Review
ABclonal: review for Histone H3 Rabbit pAb(A2348)

You may also interested in:

Overview

Product name Histone H3 Rabbit pAb
Catalog No. A2348
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 36-136 of human HIST3H3 (NP_003520.1).
Sequence VKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA
Gene ID 82908350
Swiss prot Q16695P68431
Synonyms H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Histone H3
Calculated MW 16kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ChIP 3μg antibody for 5μg-10μg of Chromatin
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples 293F, NIH/3T3, PC-12
Cellular location Chromosome, Nucleus
Customer validation

WB (Mouse embryonic fibroblasts, Raw264.7, Rat brain, HNSCsHomo sapiens, Homo sapiens, Mus musculus, Saccharomyces cerevisiae, Rattus norvegicus, Arabidopsis thaliana, Danio rerio, Bombyx mori Linnaeus, Plasmodium falciparum, Oryza sativa, Gallus gallus, Glycine max L, P. falciparum, Shrimp, P. xylostella, Drosophila, Sus scrofa, Zea mays, Nicotiana benthamiana, M. japonicus, Monascus ruber, Eurosta solidaginis, Penaeus japonicus, Ictalurus punctatus, Glycine max)

IF (Mouse E16.5 neocortexHomo sapiens, ocu, Mus musculus, Homo sapiens)

ChIp (Arabidopsis thaliana)

IP (Helicoverpa armigera, Homo sapiens)

IB (Homo sapiens)

IHC-P (Mus musculus)

immunostaining (Mus musculus)

IHC (Homo sapiens, Mus musculus)

ChIP-Seq (Arabidopsis thaliana)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Histone H3 Rabbit pAb images

ABclonal:Western blot - Histone H3 Rabbit pAb (A2348)}

Western blot - Histone H3 Rabbit pAb (A2348)

Western blot analysis of various lysates using Histone H3 Rabbit pAb (A2348) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)}

Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

Immunohistochemistry analysis of Histone H3 in paraffin-embedded rat lung using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)}

Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

Immunohistochemistry analysis of Histone H3 in paraffin-embedded human liver using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)}

Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

Immunohistochemistry analysis of Histone H3 in paraffin-embedded human brain using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)}

Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

Immunohistochemistry analysis of Histone H3 in paraffin-embedded mouse kidney using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Histone H3 Rabbit pAb (A2348)}

Immunofluorescence - Histone H3 Rabbit pAb (A2348)

Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse testis using Histone H3 Rabbit pAb (A2348) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Histone H3 Rabbit pAb (A2348)}

Immunofluorescence - Histone H3 Rabbit pAb (A2348)

Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat testis using Histone H3 Rabbit pAb (A2348) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Histone H3 Rabbit pAb (A2348)}

Immunoprecipitation - Histone H3 Rabbit pAb (A2348)

Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 10ug Histone H3 antibody (A2348). Western blot was performed from the immunoprecipitate using Histone H3 antibody (A2348) at a dilution of 1:30000.
ABclonal:Immunoprecipitation - Histone H3 Rabbit pAb (A2348)}

Immunoprecipitation - Histone H3 Rabbit pAb (A2348)

Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 10ug Histone H3 antibody (A2348). Western blot was performed from the immunoprecipitate using Histone H3 antibody (A2348) at a dilution of 1:30000.
ABclonal:Chromatin Immunoprecipitation - Histone H3 Rabbit pAb (A2348)}

Chromatin Immunoprecipitation - Histone H3 Rabbit pAb (A2348)

Chromatin immunoprecipitation analysis of extracts of 293T cells, using Histone H3 antibody (A2348) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Submit an review for A2348

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A2348 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on H3-4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H3-4. (Distance between topics and target gene indicate popularity.) H3-4

* Data provided by citexs.com, for reference only.

Publishing research using A2348? Please let us know so that we can cite the reference in this datasheet.

Antibodies (114)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order