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HIST3H3 Polyclonal Antibody (A2348)

Reviews (4)Publications (5) Datasheet

ABclonal:Western blot - HIST3H3 Polyclonal Antibody (A2348)
ABclonal:Immunofluorescence - HIST3H3 Polyclonal Antibody (A2348)
ABclonal:Chromatin Immunoprecipitation - HIST3H3 Polyclonal Antibody (A2348)

Overview

Product nameHIST3H3 Polyclonal Antibody
Catalog No.A2348
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human HIST3H3 (NP_003484.1).
SequenceREIRRYQKSTELLIRKLPFQRLMREIAQDFKTDLRFQSSAVMALQEACESYLVGLFEDTNLCVIHAKRVTIMPKDIQLARRIRGERA
Gene ID8290
Swiss protQ16695
SynonymsH3.4; H3/g; H3FT; H3t
Calculated MW15kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
ChIP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesJurkat, A-431, Mouse liver, Mouse testis, Rat kidney
Cellular locationChromosome, Nucleus
Customer validation

WB(Raw264.7, Rat brain, HNSCs, Human, Yeast, Mouse)

IF(Mouse E16.5 neocortex)

ABclonal:Western blot - HIST3H3 Polyclonal Antibody (A2348) }

Western blot - HIST3H3 Polyclonal Antibody (A2348)

Western blot analysis of extracts of various cell lines, using HIST3H3 antibody (A2348) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - HIST3H3 Polyclonal Antibody (A2348) }

Immunofluorescence - HIST3H3 Polyclonal Antibody (A2348)

Immunofluorescence analysis of U2OS cells using HIST3H3 antibody (A2348) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - HIST3H3 Polyclonal Antibody (A2348) }

Chromatin Immunoprecipitation - HIST3H3 Polyclonal Antibody (A2348)

Chromatin Immunoprecipitation analysis extracts of HeLa cell line, using HIST3H3 antibody (A2348) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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