Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

[KO Validated] HIRA Rabbit pAb (A8461)

KO/KDValidated

Review (1) Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - [KO Validated] HIRA Rabbit pAb (A8461)

Western blot analysis of various lysates using [KO Validated] HIRA Rabbit pAb (A8461) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] HIRA Rabbit pAb (A8461)

Western blot analysis of lysates from wild type (WT) and HIRA knockout (KO) 293T cells, using [KO Validated] HIRA Rabbit pAb (A8461) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - [KO Validated] HIRA Rabbit pAb (A8461)

Immunohistochemistry analysis of HIRA in paraffin-embedded human gastric cancer using [KO Validated] HIRA Rabbit pAb (A8461) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] HIRA Rabbit pAb (A8461)

Immunohistochemistry analysis of HIRA in paraffin-embedded human lung cancer using [KO Validated] HIRA Rabbit pAb (A8461) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] HIRA Rabbit pAb (A8461)

Immunohistochemistry analysis of HIRA in paraffin-embedded human colon carcinoma using [KO Validated] HIRA Rabbit pAb (A8461) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Review
ABclonal: review for [KO Validated] HIRA Rabbit pAb(A8461)

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Overview

Product name [KO Validated] HIRA Rabbit pAb
Catalog No. A8461
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human HIRA (NP_003316.3).
Sequence AQIIEREGWKTNMDFVGHRKAVTVVKFNPKIFKKKQKNGSSAKPSCPYCCCAVGSKDRSLSVWLTCLKRPLVVIHELFDKSIMDISWTLNGLGILVCSMDGSVAFLDFSQDELGDPLSEEEKSRIHQSTYGKSLAIMTEAQLSTAVIENPEMLKYQRRQQQQQLDQKSAATREMGSATSVAGVVNGESLEDIRKNLLKKQVETRTADGRRRITPLCIAQLDTGDFSTAFFNSIPLSGSLAGTMLSSHSSPQLLPLDSSTPNSFGASKPCTEPVVAASARPAGDSVNKDSMNATSTPAALSP
Gene ID 7290
Swiss prot P54198
Synonyms TUP1; DGCR1; TUPLE1; RA
Calculated MW 112kDa
Observed MW 112kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples 293T, HepG2, HeLa
Cellular location Nucleus, PML body
Customer validation

WB (Mouse liverMus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] HIRA Rabbit pAb images

ABclonal:Western blot - [KO Validated] HIRA Rabbit pAb (A8461)}

Western blot - [KO Validated] HIRA Rabbit pAb (A8461)

Western blot analysis of various lysates using [KO Validated] HIRA Rabbit pAb (A8461) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] HIRA Rabbit pAb (A8461)}

Western blot - [KO Validated] HIRA Rabbit pAb (A8461)

Western blot analysis of lysates from wild type (WT) and HIRA knockout (KO) 293T cells, using [KO Validated] HIRA Rabbit pAb (A8461) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - [KO Validated] HIRA Rabbit pAb (A8461)}

Immunohistochemistry - [KO Validated] HIRA Rabbit pAb (A8461)

Immunohistochemistry analysis of HIRA in paraffin-embedded human gastric cancer using [KO Validated] HIRA Rabbit pAb (A8461) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] HIRA Rabbit pAb (A8461)}

Immunohistochemistry - [KO Validated] HIRA Rabbit pAb (A8461)

Immunohistochemistry analysis of HIRA in paraffin-embedded human lung cancer using [KO Validated] HIRA Rabbit pAb (A8461) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] HIRA Rabbit pAb (A8461)}

Immunohistochemistry - [KO Validated] HIRA Rabbit pAb (A8461)

Immunohistochemistry analysis of HIRA in paraffin-embedded human colon carcinoma using [KO Validated] HIRA Rabbit pAb (A8461) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on HIRA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HIRA. (Distance between topics and target gene indicate popularity.) HIRA

* Data provided by citexs.com, for reference only.

Publishing research using A8461? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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