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HIF1A Polyclonal Antibody (A11945)

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ABclonal:Western blot - HIF1A Polyclonal Antibody (A11945)
Review
ABclonal: review for HIF1A Polyclonal Antibody(A11945)

Overview

Product nameHIF1A Polyclonal Antibody
Catalog No.A11945
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 70-260 of human HIF1A (NP_851397.1).
SequenceRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERIT
Gene ID3091
Swiss protQ16665
SynonymsHIF1A; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; hypoxia-inducible factor 1-alpha
Calculated MW82kDa/92kDa/95kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHepG2, HT-1080, A-549, Mouse kidney, Mouse skin, Rat kidney, Rat liver
Cellular locationCytoplasm, Nucleus, Nucleus speckle
Customer validation

WB(Human, Chicken, Rat)

IF(Human)

CoIP(Human)

IHC(Chicken)

ABclonal:Western blot - HIF1A Polyclonal Antibody (A11945) }

Western blot - HIF1A Polyclonal Antibody (A11945)

Western blot analysis of extracts of various cell lines, using HIF1A antibody (A11945) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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