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HDAC2 Polyclonal Antibody (A0867)

Publications (2) Datasheet

ABclonal:Western blot - HDAC2 Polyclonal Antibody (A0867)
ABclonal:Immunohistochemistry - HDAC2 Polyclonal Antibody (A0867)

Overview

Product nameHDAC2 Polyclonal Antibody
Catalog No.A0867
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 429-488 of human HDAC2 (NP_001518.3).
SequenceEGGRRNVADHKKGAKKARIEEDKKETEDKKTDVKEEDKSKDNSGEKTDTKGTKSEQLSNP
Gene ID3066
Swiss protQ92769
SynonymsHDAC2; HD2; RPD3; YAF1; histone deacetylase 2
Calculated MW51kDa/55kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesNCI-H460
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Human)

ABclonal:Western blot - HDAC2 Polyclonal Antibody (A0867) }

Western blot - HDAC2 Polyclonal Antibody (A0867)

Western blot analysis of extracts of H460 cells, using HDAC2 antibody (A0867) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - HDAC2 Polyclonal Antibody (A0867) }

Immunohistochemistry - HDAC2 Polyclonal Antibody (A0867)

Immunohistochemistry of paraffin-embedded human lung cancer using HDAC2 antibody (A0867) at dilution of 1:100 (40x lens).

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