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HDAC1 Polyclonal Antibody (A0238)

Reviews (5)Publications (2) Datasheet

ABclonal:Western blot - HDAC1 Polyclonal Antibody (A0238)
ABclonal:Western blot - HDAC1 Polyclonal Antibody (A0238)
ABclonal:Immunohistochemistry - HDAC1 Polyclonal Antibody (A0238)
ABclonal:Immunohistochemistry - HDAC1 Polyclonal Antibody (A0238)
ABclonal:Immunohistochemistry - HDAC1 Polyclonal Antibody (A0238)
ABclonal:Immunohistochemistry - HDAC1 Polyclonal Antibody (A0238)
ABclonal:Immunofluorescence - HDAC1 Polyclonal Antibody (A0238)
ABclonal:Immunoprecipitation - HDAC1 Polyclonal Antibody (A0238)

Overview

Product nameHDAC1 Polyclonal Antibody
Catalog No.A0238
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
ImmunogenA synthetic peptide of human HDAC1
SequenceEmail for sequence
Gene ID3065
Swiss protQ13547
SynonymsGON-10; HD1; RPD3; RPD3L1
Calculated MW55kDa
Observed MW62kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T, HeLa, HepG2, MCF7, Mouse thymus, Mouse testis, Rat thymus, Rat testis
Cellular locationNucleus
Customer validation

IF(Human acute leukemia cells)

WB(Human acute leukemia cells, HL60,Hut102, 293T, Rat)

IP(Human acute leukemia cells)

IP(Rat)

ChIP(Rat)

ABclonal:Western blot - HDAC1 Polyclonal Antibody (A0238) }

Western blot - HDAC1 Polyclonal Antibody (A0238)

Western blot analysis of extracts of various cell lines, using HDAC1 antibody (A0238) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - HDAC1 Polyclonal Antibody (A0238) }

Western blot - HDAC1 Polyclonal Antibody (A0238)

Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (A0238) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - HDAC1 Polyclonal Antibody (A0238) }

Immunohistochemistry - HDAC1 Polyclonal Antibody (A0238)

Immunohistochemistry of paraffin-embedded human lung cancer using HDAC1 antibody (A0238) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - HDAC1 Polyclonal Antibody (A0238) }

Immunohistochemistry - HDAC1 Polyclonal Antibody (A0238)

Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC1 antibody (A0238) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - HDAC1 Polyclonal Antibody (A0238) }

Immunohistochemistry - HDAC1 Polyclonal Antibody (A0238)

Immunohistochemistry of paraffin-embedded human esophagus using HDAC1 antibody (A0238) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - HDAC1 Polyclonal Antibody (A0238) }

Immunohistochemistry - HDAC1 Polyclonal Antibody (A0238)

Immunohistochemistry of paraffin-embedded human stomach using HDAC1 antibody (A0238) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - HDAC1 Polyclonal Antibody (A0238) }

Immunofluorescence - HDAC1 Polyclonal Antibody (A0238)

Immunofluorescence analysis of A549 cells using HDAC1 antibody (A0238). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - HDAC1 Polyclonal Antibody (A0238) }

Immunoprecipitation - HDAC1 Polyclonal Antibody (A0238)

Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug HDAC1 antibody (A0238). Western blot was performed from the immunoprecipitate using HDAC1 antibody (A0238) at a dilition of 1:1000.

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