Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

USP7/HAUSP Rabbit pAb (A13564)

Publications (6) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - USP7/HAUSP Rabbit pAb (A13564)

Western blot analysis of extracts of various cell lines, using USP7/HAUSP antibody (A13564) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - USP7/HAUSP Rabbit pAb (A13564)

Immunohistochemistry analysis of paraffin-embedded human kidney cancer using USP7/HAUSP antibody (A13564) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - USP7/HAUSP Rabbit pAb (A13564)

Immunohistochemistry analysis of paraffin-embedded mouse heart using USP7/HAUSP antibody (A13564) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - USP7/HAUSP Rabbit pAb (A13564)

Immunofluorescence analysis of HeLa cells using USP7/HAUSP antibody (A13564).

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Overview

Product name USP7/HAUSP Rabbit pAb
Catalog No. A13564
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 206-1102 of human USP7/HAUSP (NP_003461.2).
Sequence DSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEVLQAVTDHDIPQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAEDQFCGHQGNDMYDEEKVKYTVFKVLKNSSLAEFVQSLSQTMGFPQDQIRLWPMQARSNGTKRPAMLDNEADGNKTMIELSDNENPWTIFLETVDPELAASGATLPKFDKDHDVMLFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCDRAGFIQDTSLILYEEVKPNLTERIQDYDVSLDKALDELMDGDIIVFQKDDPENDNSELPTAKEYFRDLYHRVDVIFCDKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTDPMLLQFFKSQGYRDGPGNPLRHNYEGTLRDLLQFFKPRQPKKLYYQQLKMKITDFENRRSFKCIWLNSQFREEEITLYPDKHGCVRDLLEECKKAVELGEKASGKLRLLEIVSYKIIGVHQEDELLECLSPATSRTFRIEEIPLDQVDIDKENEMLVTVAHFHKEVFGTFGIPFLLRIHQGEHFREVMKRIQSLLDIQEKEFEKFKFAIVMMGRHQYINEDEYEVNLKDFEPQPGNMSHPRPWLGLDHFNKAPKRSRYTYLEKAIKIHN
Gene ID 7874
Swiss prot Q93009
Synonyms TEF1; HAUSP; HAFOUS; DEL16P13.2; C16DELp13.2; USP7/HAUSP
Calculated MW 128kDa
Observed MW 138kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HepG2, Rat testis
Cellular location Cytoplasm, Nucleus, PML body
Customer validation

WB (Homo sapiens)

IHC (Homo sapiens, Mus musculus)

IF (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

USP7/HAUSP Rabbit pAb images

ABclonal:Western blot - USP7/HAUSP Rabbit pAb (A13564)}

Western blot - USP7/HAUSP Rabbit pAb (A13564)

Western blot analysis of extracts of various cell lines, using USP7/HAUSP antibody (A13564) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - USP7/HAUSP Rabbit pAb (A13564)}

Immunohistochemistry - USP7/HAUSP Rabbit pAb (A13564)

Immunohistochemistry analysis of paraffin-embedded human kidney cancer using USP7/HAUSP antibody (A13564) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - USP7/HAUSP Rabbit pAb (A13564)}

Immunohistochemistry - USP7/HAUSP Rabbit pAb (A13564)

Immunohistochemistry analysis of paraffin-embedded mouse heart using USP7/HAUSP antibody (A13564) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - USP7/HAUSP Rabbit pAb (A13564)}

Immunofluorescence - USP7/HAUSP Rabbit pAb (A13564)

Immunofluorescence analysis of HeLa cells using USP7/HAUSP antibody (A13564).

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Related Targets

Based on existing publications, the following genes are closely related to research on USP7. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to USP7. (Distance between topics and target gene indicate popularity.) USP7

* Data provided by citexs.com, for reference only.

Publishing research using A13564? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Proteins (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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