Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

GTSE1 Rabbit pAb (A13902)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - GTSE1 Rabbit pAb (A13902)

Western blot analysis of various lysates using GTSE1 Rabbit pAb (A13902) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Overview

Product name GTSE1 Rabbit pAb
Catalog No. A13902
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 30-290 of human GTSE1 (NP_057510.4).
Sequence LLADEKFDFDLSLSSSSANEDDEVFFGPFGHKERCIAASLELNNPVPEQPPLPTSESPFAWSPLAGEKFVEVYKEAHLLALHIESSSRNQAAQAAKPEDPRSQGVERFIQESKLKINLFEKEKEMKKSPTSLKRETYYLSDSPLLGPPVGEPRLLASSPALPSSGAQARLTRAPGPPHSAHALPRESCTAHAASQAATQRKPGTKLLLPRAASVRGRSIPGAAEKPKKEIPASPSRTKIPAEKESHRDVLPDKPAPGAVNV
Gene ID 51512
Swiss prot Q9NYZ3
Synonyms B99; GTSE1
Calculated MW 77kDa
Observed MW 77kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples LO2, U-87MG, HeLa, Mouse spleen, Rat spleen
Cellular location Cytoplasm, cytoskeleton

Documents

Certificate of Compliance

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Lot number

Research Area

GTSE1 Rabbit pAb images

ABclonal:Western blot - GTSE1 Rabbit pAb (A13902)}

Western blot - GTSE1 Rabbit pAb (A13902)

Western blot analysis of various lysates using GTSE1 Rabbit pAb (A13902) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on GTSE1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GTSE1. (Distance between topics and target gene indicate popularity.) GTSE1

* Data provided by citexs.com, for reference only.

Publishing research using A13902? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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