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GRIA1 Polyclonal Antibody (A1826)

Publication (1) Datasheet

ABclonal:Western blot - GRIA1 Polyclonal Antibody (A1826)
ABclonal:Immunofluorescence - GRIA1 Polyclonal Antibody (A1826)
ABclonal:Immunofluorescence - GRIA1 Polyclonal Antibody (A1826)

Overview

Product nameGRIA1 Polyclonal Antibody
Catalog No.A1826
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 19-300 of human GRIA1 (NP_000818.2).
SequenceANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQDALISIIDHYKWQKFVYIYDADRGLSVLQKVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIIKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPAKIMQQWKNSDARDHTRVDWKRPKYTSALTYDGVKVM
Gene ID2890
Swiss protP42261
SynonymsGRIA1; GLUH1; GLUR1; GLURA; GluA1; HBGR1; glutamate receptor 1
Calculated MW92kDa/93kDa/101kDa/102kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesBT-474
Cellular locationCell junction, Cell membrane, Cell projection, Endoplasmic reticulum membrane, Multi-pass membrane protein, dendrite, dendritic spine, postsynaptic cell membrane, postsynaptic density, synapse
Customer validation

WB(Mouse)

ABclonal:Western blot - GRIA1 Polyclonal Antibody (A1826) }

Western blot - GRIA1 Polyclonal Antibody (A1826)

Western blot analysis of extracts of BT-474 cells, using GRIA1 antibody (A1826) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - GRIA1 Polyclonal Antibody (A1826) }

Immunofluorescence - GRIA1 Polyclonal Antibody (A1826)

Immunofluorescence analysis of rat brain using GRIA1 antibody (A1826) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GRIA1 Polyclonal Antibody (A1826) }

Immunofluorescence - GRIA1 Polyclonal Antibody (A1826)

Immunofluorescence analysis of mouse brain using GRIA1 antibody (A1826) at dilution of 1:100. Blue: DAPI for nuclear staining.

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