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GPC1 Rabbit pAb (A13019)

Review (1) Datasheet

ABclonal:Western blot - GPC1 Polyclonal Antibody (A13019)

Western blot analysis of extracts of various cell lines, using GPC1 antibody (A13019) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Review
ABclonal: review for GPC1 Rabbit pAb(A13019)

Overview

Product nameGPC1 Rabbit pAb
Catalog No.A13019
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 290-420 of human GPC1 (NP_002072.2).
SequenceWRNLLDSMVLITDKFWGTSGVESVIGSVHTWLAEAINALQDNRDTLTAKVIQGCGNPKVNPQGPGPEEKRRRGKLAPRERPPSGTLEKLVSEAKAQLRDVQDFWISLPGTLCSEKMALSTASDDRCWNGMA
Gene ID2817
Swiss protP35052
SynonymsGPC1; glypican
Calculated MW31kDa/61kDa
Observed MW62kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesNIH/3T3, Mouse brain, Rat brain
Cellular locationCell membrane, Endosome, Extracellular side, GPI-anchor, Lipid-anchor, Secreted, extracellular space
Customer validation

(Mouse liver)

ABclonal:Western blot - GPC1 Polyclonal Antibody (A13019) }

Western blot - GPC1 Polyclonal Antibody (A13019)

Western blot analysis of extracts of various cell lines, using GPC1 antibody (A13019) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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