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GJA1 Rabbit pAb (A2163)

Publication (1) Datasheet

ABclonal:Western blot - GJA1 Polyclonal Antibody (A2163)

Overview

Product nameGJA1 Rabbit pAb
Catalog No.A2163
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 233-382 of human GJA1 (NP_000156.1).
SequenceFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI
Gene ID2697
Swiss protP17302
SynonymsGJA1; AVSD3; CMDR; CX43; EKVP; GJAL; HLHS1; HSS; ODDD; PPKCA; EKVP3
Calculated MW43kDa
Observed MW50kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse heart
Cellular locationCell junction, Cell membrane, Endoplasmic reticulum, Multi-pass membrane protein, gap junction
Customer validation

WB(Rattus norvegicus)

ABclonal:Western blot - GJA1 Polyclonal Antibody (A2163) }

Western blot - GJA1 Polyclonal Antibody (A2163)

Western blot analysis of extracts of mouse heart, using GJA1 antibody (A2163) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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