Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

GCLC Rabbit pAb (A1038)

Publications (12) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - GCLC Rabbit pAb (A1038)

Western blot analysis of various lysates, using GCLC Rabbit pAb (A1038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - GCLC Rabbit pAb (A1038)

Immunofluorescence analysis of Jurkat cells using GCLC Rabbit pAb (A1038) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GCLC Rabbit pAb (A1038)

Immunofluorescence analysis of PC-12 cells using GCLC Rabbit pAb (A1038) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name GCLC Rabbit pAb
Catalog No. A1038
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human GCLC (NP_001489.1).
Sequence MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQV
Gene ID 2729
Swiss prot P48506
Synonyms GCL; GCS; GLCL; GLCLC; GCLC
Calculated MW 73kDa
Observed MW 73kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:3000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, Mouse liver
Cellular location cytosol, glutamate-cysteine ligase complex, mitochondrion
Customer validation

WB (Struthio camelus, Mus musculus, Gallus gallus, Rattus norvegicus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

GCLC Rabbit pAb images

ABclonal:Western blot - GCLC Rabbit pAb (A1038)}

Western blot - GCLC Rabbit pAb (A1038)

Western blot analysis of various lysates, using GCLC Rabbit pAb (A1038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - GCLC Rabbit pAb (A1038)}

Immunofluorescence - GCLC Rabbit pAb (A1038)

Immunofluorescence analysis of Jurkat cells using GCLC Rabbit pAb (A1038) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GCLC Rabbit pAb (A1038)}

Immunofluorescence - GCLC Rabbit pAb (A1038)

Immunofluorescence analysis of PC-12 cells using GCLC Rabbit pAb (A1038) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on GCLC. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GCLC. (Distance between topics and target gene indicate popularity.) GCLC

* Data provided by citexs.com, for reference only.

Publishing research using A1038? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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