Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

GAS2L1 Rabbit pAb (A2143)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - GAS2L1 Rabbit pAb (A2143)

Western blot analysis of extracts of various cell lines, using GAS2L1 antibody (A2143) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name GAS2L1 Rabbit pAb
Catalog No. A2143
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the growth arrest-specific 2 protein family. This protein binds components of the cytoskeleton and may be involved in mediating interactions between microtubules and microfilaments. This protein localizes to the proximal end of mature centrioles and links centrosomes to both microtubules and actin. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 9.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 130-400 of human GAS2L1 (NP_006469.2).
Sequence RKNEKSVVLCLLEVARRGARLGLLAPRLVQFEQEIERELRAAPPAPNAPAAGEDTTETAPAPGTPARGPRMTPSDLRNLDELVREILGRCTCPDQFPMIKVSEGKYRVGDSSLLIFVRVLRSHVMVRVGGGWDTLEHYLDKHDPCRCSSTAHRPPQPRVCTFSPQRVSPTTSPRPASPVPGSERRGSRPEMTPVSLRSTKEGPETPPRPRDQLPPHPRSRRYSGDSDSSASSAQSGPLGTRSDDTGTGPRRERPSRRLTTGTPASPRRPPA
Gene ID 10634
Swiss prot Q99501
Synonyms GAR22; GAS2L1
Calculated MW 73kDa
Observed MW 58kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples A673, 22Rv1
Cellular location Cytoplasm, cytoskeleton

Research Area

GAS2L1 Rabbit pAb images

ABclonal:Western blot - GAS2L1 Rabbit pAb (A2143)}

Western blot - GAS2L1 Rabbit pAb (A2143)

Western blot analysis of extracts of various cell lines, using GAS2L1 antibody (A2143) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on GAS2L1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GAS2L1. (Distance between topics and target gene indicate popularity.) GAS2L1

* Data provided by citexs.com, for reference only.

Publishing research using A2143? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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