Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

GABARAP Rabbit pAb (A13855)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - GABARAP Rabbit pAb (A13855)

Western blot analysis of extracts of various cell lines, using GABARAP antibody (A13855) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.

ABclonal:Immunofluorescence - GABARAP Rabbit pAb (A13855)

Immunofluorescence analysis of HeLa cells using GABARAP antibody (A13855).

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Overview

Product name GABARAP Rabbit pAb
Catalog No. A13855
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GABARAP (NP_009209.1).
Sequence MKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHE
Gene ID 11337
Swiss prot O95166
Synonyms MM46; ATG8A; GABARAP-a; GABARAP
Calculated MW 14kDa
Observed MW 14kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples SH-SY5Y, Mouse brain, rat kidney
Cellular location Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Golgi apparatus membrane, autophagosome, cytoskeleton

Documents

Certificate of Compliance

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Research Area

GABARAP Rabbit pAb images

ABclonal:Western blot - GABARAP Rabbit pAb (A13855)}

Western blot - GABARAP Rabbit pAb (A13855)

Western blot analysis of extracts of various cell lines, using GABARAP antibody (A13855) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.
ABclonal:Immunofluorescence - GABARAP Rabbit pAb (A13855)}

Immunofluorescence - GABARAP Rabbit pAb (A13855)

Immunofluorescence analysis of HeLa cells using GABARAP antibody (A13855).

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Related Targets

Based on existing publications, the following genes are closely related to research on GABARAP. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GABARAP. (Distance between topics and target gene indicate popularity.) GABARAP

* Data provided by citexs.com, for reference only.

Publishing research using A13855? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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