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Fatty Acid Synthase (FASN) Rabbit pAb (A6273)

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ABclonal:Western blot - Fatty Acid Synthase (FASN) Rabbit pAb (A6273)

Western blot analysis of extracts of various cell lines, using Fatty Acid Synthase (FASN) antibody (A6273).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Review
ABclonal: review for Fatty Acid Synthase (FASN) Rabbit pAb(A6273)

Overview

Product nameFatty Acid Synthase (FASN) Rabbit pAb
Catalog No.A6273
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
ImmunogenA synthetic peptide of human Fatty Acid Synthase (FASN)
SequenceEmail for sequence
Gene ID2194
Swiss protP49327
SynonymsFAS; OA-519; SDR27X1; FASN
Calculated MW273kDa
Observed MW270kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesRaji, HeLa
Cellular locationCytoplasm, Melanosome
Customer validation

(Mouse liver)

WB(Homo sapiens, Mus musculus)

IHC(Homo sapiens)

ABclonal:Western blot - Fatty Acid Synthase (FASN) Rabbit pAb (A6273) }

Western blot - Fatty Acid Synthase (FASN) Rabbit pAb (A6273)

Western blot analysis of extracts of various cell lines, using Fatty Acid Synthase (FASN) antibody (A6273).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

* For research use only. Not for therapeutic or diagnostic purposes.

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