Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Ferritin Light Chain Rabbit pAb (A1768)

Publications (3) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Ferritin Light Chain Rabbit pAb (A1768)

Western blot analysis of extracts of various cell lines, using Ferritin Light Chain antibody (A1768) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Ferritin Light Chain Rabbit pAb (A1768)

Western blot analysis of extracts of Rat liver, using Ferritin Light Chain antibody (A1768) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded human colon using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded human liver using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded human lung using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name Ferritin Light Chain Rabbit pAb
Catalog No. A1768
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human Ferritin Light Chain (NP_000137.2).
Sequence MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD
Gene ID 2512
Swiss prot P02792
Synonyms LFTD; NBIA3; Ferritin Light Chain
Calculated MW 20kDa
Observed MW 20kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Mouse liver, Mouse brain, Rat liver
Cellular location cytoplasm, cytosol, extracellular exosome, extracellular region
Customer validation

WB (Homo sapiens, Mus musculus)

IF (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Ferritin Light Chain Rabbit pAb images

ABclonal:Western blot - Ferritin Light Chain Rabbit pAb (A1768)}

Western blot - Ferritin Light Chain Rabbit pAb (A1768)

Western blot analysis of extracts of various cell lines, using Ferritin Light Chain antibody (A1768) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - Ferritin Light Chain Rabbit pAb (A1768)}

Western blot - Ferritin Light Chain Rabbit pAb (A1768)

Western blot analysis of extracts of Rat liver, using Ferritin Light Chain antibody (A1768) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)}

Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded human colon using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)}

Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded human liver using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)}

Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded human lung using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)}

Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)}

Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)}

Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)}

Immunohistochemistry - Ferritin Light Chain Rabbit pAb (A1768)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Ferritin Light Chain Rabbit pAb (A1768) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on FTL. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to FTL. (Distance between topics and target gene indicate popularity.) FTL

* Data provided by citexs.com, for reference only.

Publishing research using A1768? Please let us know so that we can cite the reference in this datasheet.

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