Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Ferritin Heavy Chain Rabbit pAb (A1144)

Publications (19) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Ferritin Heavy Chain Rabbit pAb (A1144)

Western blot analysis of various lysates using Ferritin Heavy Chain Rabbit pAb (A1144) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Ferritin Heavy Chain Rabbit pAb (A1144)

Immunohistochemistry analysis of Ferritin Heavy Chain in paraffin-embedded human liver tissue using Ferritin Heavy Chain Rabbit pAb (A1144) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Ferritin Heavy Chain Rabbit pAb (A1144)

Immunohistochemistry analysis of Ferritin Heavy Chain in paraffin-embedded mouse kidney tissue using Ferritin Heavy Chain Rabbit pAb (A1144) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Ferritin Heavy Chain Rabbit pAb (A1144)

Immunohistochemistry analysis of Ferritin Heavy Chain in paraffin-embedded rat kidney tissue using Ferritin Heavy Chain Rabbit pAb (A1144) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Ferritin Heavy Chain Rabbit pAb (A1144)

Immunofluorescence analysis of A-549 cells using Ferritin Heavy Chain Rabbit pAb (A1144) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name Ferritin Heavy Chain Rabbit pAb
Catalog No. A1144
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human Ferritin Heavy Chain (NP_002023.2).
Sequence MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES
Gene ID 2495
Swiss prot P02794
Synonyms FHC; FTH; HFE5; PLIF; FTHL6; PIG15; Ferritin Heavy Chain
Calculated MW 21kDa
Observed MW 21kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples RAW 264.7, C6
Cellular location cytoplasm, cytosol, extracellular exosome, extracellular region, nucleus
Customer validation

WB (Homo sapiens, Mus musculus, Danio rerio)

IHC (Homo sapiens, mus musculus, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Ferritin Heavy Chain Rabbit pAb images

ABclonal:Western blot - Ferritin Heavy Chain Rabbit pAb (A1144)}

Western blot - Ferritin Heavy Chain Rabbit pAb (A1144)

Western blot analysis of various lysates using Ferritin Heavy Chain Rabbit pAb (A1144) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Ferritin Heavy Chain Rabbit pAb (A1144)}

Immunohistochemistry - Ferritin Heavy Chain Rabbit pAb (A1144)

Immunohistochemistry analysis of Ferritin Heavy Chain in paraffin-embedded human liver tissue using Ferritin Heavy Chain Rabbit pAb (A1144) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Ferritin Heavy Chain Rabbit pAb (A1144)}

Immunohistochemistry - Ferritin Heavy Chain Rabbit pAb (A1144)

Immunohistochemistry analysis of Ferritin Heavy Chain in paraffin-embedded mouse kidney tissue using Ferritin Heavy Chain Rabbit pAb (A1144) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Ferritin Heavy Chain Rabbit pAb (A1144)}

Immunohistochemistry - Ferritin Heavy Chain Rabbit pAb (A1144)

Immunohistochemistry analysis of Ferritin Heavy Chain in paraffin-embedded rat kidney tissue using Ferritin Heavy Chain Rabbit pAb (A1144) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Ferritin Heavy Chain Rabbit pAb (A1144)}

Immunofluorescence - Ferritin Heavy Chain Rabbit pAb (A1144)

Immunofluorescence analysis of A-549 cells using Ferritin Heavy Chain Rabbit pAb (A1144) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on FTH1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to FTH1. (Distance between topics and target gene indicate popularity.) FTH1

* Data provided by citexs.com, for reference only.

Publishing research using A1144? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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