Products > Catalog Antibodies > Polyclonal Antibodies

FN1 Polyclonal Antibody (A7488)

Publications (2) Datasheet

ABclonal:Western blot - FN1 Polyclonal Antibody (A7488)
ABclonal:Immunohistochemistry - FN1 Polyclonal Antibody (A7488)
ABclonal:Immunohistochemistry - FN1 Polyclonal Antibody (A7488)
ABclonal:Immunohistochemistry - FN1 Polyclonal Antibody (A7488)

Overview

Product nameFN1 Polyclonal Antibody
Catalog No.A7488
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human FN1 (NP_002017.1).
SequenceMLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGA
Gene ID2335
Swiss protP02751
SynonymsCIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF
Calculated MW71-73kDa/221-272kDa
Observed MW290kDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesNIH/3T3
Cellular locationSecreted, extracellular matrix, extracellular space
Customer validation

WB(Rat)

IF(Human)

ABclonal:Western blot - FN1 Polyclonal Antibody (A7488) }

Western blot - FN1 Polyclonal Antibody (A7488)

Western blot analysis of extracts of NIH/3T3 cells, using FN1 antibody (A7488) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - FN1 Polyclonal Antibody (A7488) }

Immunohistochemistry - FN1 Polyclonal Antibody (A7488)

Immunohistochemistry of paraffin-embedded rat brain using FN1 Antibody (A7488) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - FN1 Polyclonal Antibody (A7488) }

Immunohistochemistry - FN1 Polyclonal Antibody (A7488)

Immunohistochemistry of paraffin-embedded mouse brain using FN1 Antibody (A7488) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - FN1 Polyclonal Antibody (A7488) }

Immunohistochemistry - FN1 Polyclonal Antibody (A7488)

Immunohistochemistry of paraffin-embedded mouse kidney using FN1 Antibody (A7488) at dilution of 1:100 (40x lens).

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