Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

[KO Validated] Flotillin 1 Rabbit pAb (A6220)

KO/KDValidated

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] Flotillin 1 Rabbit pAb (A6220)

Western blot analysis of lysates from wild type (WT) and Flotillin 1 knockout (KO) 293T cells, using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] Flotillin 1 Rabbit pAb (A6220)

Western blot analysis of various lysates, using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] Flotillin 1 Rabbit pAb (A6220)

Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded rat heart using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] Flotillin 1 Rabbit pAb (A6220)

Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded mouse kidney using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] Flotillin 1 Rabbit pAb (A6220)

Immunofluorescence analysis of NIH/3T3 cells using Flotillin 1 Rabbit pAb (A6220) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] Flotillin 1 Rabbit pAb (A6220)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg Flotillin 1 antibody (A6220). Western blot was performed from the immunoprecipitate using Flotillin 1 antibody (A6220) at a dilution of 1:1000.

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Overview

Product name [KO Validated] Flotillin 1 Rabbit pAb
Catalog No. A6220
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 155-427 of human Flotillin 1 (NP_005794.1).
Sequence HDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA
Gene ID 10211
Swiss prot O75955
Synonyms FLOT1; 1
Calculated MW 47kDa
Observed MW 47kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples 293T, HeLa, A-549, Mouse brain, Rat brain
Cellular location Cell membrane, Endosome, Melanosome, Membrane, Peripheral membrane protein, caveola
Customer validation

WB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] Flotillin 1 Rabbit pAb images

ABclonal:Western blot - [KO Validated] Flotillin 1 Rabbit pAb (A6220)}

Western blot - [KO Validated] Flotillin 1 Rabbit pAb (A6220)

Western blot analysis of lysates from wild type (WT) and Flotillin 1 knockout (KO) 293T cells, using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] Flotillin 1 Rabbit pAb (A6220)}

Western blot - [KO Validated] Flotillin 1 Rabbit pAb (A6220)

Western blot analysis of various lysates, using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] Flotillin 1 Rabbit pAb (A6220)}

Immunohistochemistry - [KO Validated] Flotillin 1 Rabbit pAb (A6220)

Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded rat heart using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] Flotillin 1 Rabbit pAb (A6220)}

Immunohistochemistry - [KO Validated] Flotillin 1 Rabbit pAb (A6220)

Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded mouse kidney using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] Flotillin 1 Rabbit pAb (A6220)}

Immunofluorescence - [KO Validated] Flotillin 1 Rabbit pAb (A6220)

Immunofluorescence analysis of NIH/3T3 cells using Flotillin 1 Rabbit pAb (A6220) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] Flotillin 1 Rabbit pAb (A6220)}

Immunoprecipitation - [KO Validated] Flotillin 1 Rabbit pAb (A6220)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg Flotillin 1 antibody (A6220). Western blot was performed from the immunoprecipitate using Flotillin 1 antibody (A6220) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on FLOT1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to FLOT1. (Distance between topics and target gene indicate popularity.) FLOT1

* Data provided by citexs.com, for reference only.

Publishing research using A6220? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

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Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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