Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Ambra1 Rabbit pAb (A1083)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Ambra1 Rabbit pAb (A1083)

Western blot analysis of PANC-1, using Ambra1 antibody (A1083) at 1:800 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Ambra1 Rabbit pAb (A1083)

Immunohistochemistry analysis of paraffin-embedded rat lung using AMBRA1 antibody (A1083) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Ambra1 Rabbit pAb (A1083)

Immunohistochemistry analysis of paraffin-embedded mouse lung using AMBRA1 antibody (A1083) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Ambra1 Rabbit pAb (A1083)

Immunofluorescence analysis of NIH/3T3 cells using Ambra1 Rabbit pAb (A1083) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name Ambra1 Rabbit pAb
Catalog No. A1083
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Enables GTPase binding activity and ubiquitin protein ligase binding activity. Involved in macroautophagy; positive regulation of phosphatidylinositol 3-kinase activity; and response to mitochondrial depolarisation. Located in cytosol. Colocalizes with mitochondrion. Biomarker of multiple system atrophy.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human Ambra1 (NP_060219.2).
Sequence GAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR
Gene ID 55626
Swiss prot Q9C0C7
Synonyms DCAF3; WDR94; Ambra1
Calculated MW 143kDa
Observed MW 130-150kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples PANC-1
Cellular location Cytoplasmic vesicle, autophagosome
Customer validation

IF (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Ambra1 Rabbit pAb images

ABclonal:Western blot - Ambra1 Rabbit pAb (A1083)}

Western blot - Ambra1 Rabbit pAb (A1083)

Western blot analysis of PANC-1, using Ambra1 antibody (A1083) at 1:800 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Ambra1 Rabbit pAb (A1083)}

Immunohistochemistry - Ambra1 Rabbit pAb (A1083)

Immunohistochemistry analysis of paraffin-embedded rat lung using AMBRA1 antibody (A1083) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Ambra1 Rabbit pAb (A1083)}

Immunohistochemistry - Ambra1 Rabbit pAb (A1083)

Immunohistochemistry analysis of paraffin-embedded mouse lung using AMBRA1 antibody (A1083) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Ambra1 Rabbit pAb (A1083)}

Immunofluorescence - Ambra1 Rabbit pAb (A1083)

Immunofluorescence analysis of NIH/3T3 cells using Ambra1 Rabbit pAb (A1083) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on AMBRA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to AMBRA1. (Distance between topics and target gene indicate popularity.) AMBRA1

* Data provided by citexs.com, for reference only.

Publishing research using A1083? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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