Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

VEGF-D Rabbit pAb (A1194)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - VEGF-D Rabbit pAb (A1194)

Western blot analysis of extracts of NCI-H460 cells, using VEGFD antibody (A1194) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name VEGF-D Rabbit pAb
Catalog No. A1194
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C. Read-through transcription has been observed between this locus and the upstream PIR (GeneID 8544) locus.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 20-220 of human VEGF-D (NP_004460.1).
Sequence QGSSNEHGPVKRSSQSTLERSEQQIRAASSLEELLRITHSEDWKLWRCRLRLKSFTSMDSRSASHRSTRFAATFYDIETLKVIDEEWQRTQCSPRETCVEVASELGKSTNTFFKPPCVNVFRCGGCCNEESLICMNTSTSYISKQLFEISVPLTSVPELVPVKVANHTGCKCLPTAPRHPYSIIRRSIQIPEEDRCSHSKK
Gene ID 2277
Swiss prot O43915
Synonyms FIGF; VEGF-D
Calculated MW 40kDa
Observed MW 50kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples NCI-H460
Cellular location Secreted

Research Area

VEGF-D Rabbit pAb images

ABclonal:Western blot - VEGF-D Rabbit pAb (A1194)}

Western blot - VEGF-D Rabbit pAb (A1194)

Western blot analysis of extracts of NCI-H460 cells, using VEGFD antibody (A1194) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on VEGFD. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to VEGFD. (Distance between topics and target gene indicate popularity.) VEGFD

* Data provided by citexs.com, for reference only.

Publishing research using A1194? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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