Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

FCAR Rabbit pAb (A8888)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - FCAR Rabbit pAb (A8888)

Western blot analysis of lysates from 293T cells, using FCAR Rabbit pAb (A8888).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name FCAR Rabbit pAb
Catalog No. A8888
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 60-287 of human FCAR (NP_001991.1).
Sequence LMIIKNSTYREIGRRLKFWNETDPEFVIDHMDANKAGRYQCQYRIGHYRFRYSDTLELVVTGLYGKPFLSADRGLVLMPGENISLTCSSAHIPFDRFSLAKEGELSLPQHQSGEHPANFSLGPVDLNVSGIYRCYGWYNRSPYLWSFPSNALELVVTDSIHQDYTTQNLIRMAVAGLVLVALLAILVENWHSHTALNKEASADVAEPSWSQQMCQPGLTFARTPSVCK
Gene ID 2204
Swiss prot P24071
Synonyms CD89; FcalphaR; FcalphaRI; CTB-61M7.2; FCAR
Calculated MW 32kDa
Observed MW 40kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:200 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 293T
Cellular location Cell membrane, Secreted, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Lot number

Research Area

FCAR Rabbit pAb images

ABclonal:Western blot - FCAR Rabbit pAb (A8888)}

Western blot - FCAR Rabbit pAb (A8888)

Western blot analysis of lysates from 293T cells, using FCAR Rabbit pAb (A8888).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on FCAR. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to FCAR. (Distance between topics and target gene indicate popularity.) FCAR

* Data provided by citexs.com, for reference only.

Publishing research using A8888? Please let us know so that we can cite the reference in this datasheet.

ELISA Kits (1)

Proteins (1)

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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