Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

FBLN5 Rabbit pAb (A9961)

Publications (3) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - FBLN5 Rabbit pAb (A9961)

Western blot analysis of extracts of various cell lines, using FBLN5 antibody (A9961) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - FBLN5 Rabbit pAb (A9961)

Western blot analysis of extracts of A-431 cells, using FBLN5 antibody (A9961) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - FBLN5 Rabbit pAb (A9961)

Immunofluorescence analysis of L929 cells using FBLN5 Polyclonal Antibody (A9961) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name FBLN5 Rabbit pAb
Catalog No. A9961
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a secreted, extracellular matrix protein containing an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. It is prominently expressed in developing arteries but less so in adult vessels. However, its expression is reinduced in balloon-injured vessels and atherosclerotic lesions, notably in intimal vascular smooth muscle cells and endothelial cells. Therefore, the protein encoded by this gene may play a role in vascular development and remodeling. Defects in this gene are a cause of autosomal dominant cutis laxa, autosomal recessive cutis laxa type I (CL type I), and age-related macular degeneration type 3 (ARMD3).

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 199-448 of human FBLN5 (NP_006320.2).
Sequence NEDGRSCQDVNECATENPCVQTCVNTYGSFICRCDPGYELEEDGVHCSDMDECSFSEFLCQHECVNQPGTYFCSCPPGYILLDDNRSCQDINECEHRNHTCNLQQTCYNLQGGFKCIDPIRCEEPYLRISDNRCMCPAENPGCRDQPFTILYRDMDVVSGRSVPADIFQMQATTRYPGAYYIFQIKSGNEGREFYMRQTGPISATLVMTRPIKGPREIQLDLEMITVNTVINFRGSSVIRLRIYVSQYPF
Gene ID 10516
Swiss prot Q9UBX5
Synonyms EVEC; UP50; ADCL2; ARMD3; CMT1H; DANCE; ARCL1A; FIBL-5; HNARMD; FBLN5
Calculated MW 50kDa
Observed MW 60kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples A-431, Mouse lung, Rat lung
Cellular location Secreted, extracellular matrix, extracellular space
Customer validation

WB (Danio rerio, Mus musculus)

IHC (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

FBLN5 Rabbit pAb images

ABclonal:Western blot - FBLN5 Rabbit pAb (A9961)}

Western blot - FBLN5 Rabbit pAb (A9961)

Western blot analysis of extracts of various cell lines, using FBLN5 antibody (A9961) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - FBLN5 Rabbit pAb (A9961)}

Western blot - FBLN5 Rabbit pAb (A9961)

Western blot analysis of extracts of A-431 cells, using FBLN5 antibody (A9961) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - FBLN5 Rabbit pAb (A9961)}

Immunofluorescence - FBLN5 Rabbit pAb (A9961)

Immunofluorescence analysis of L929 cells using FBLN5 Polyclonal Antibody (A9961) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on FBLN5. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to FBLN5. (Distance between topics and target gene indicate popularity.) FBLN5

* Data provided by citexs.com, for reference only.

Publishing research using A9961? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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