Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Fas Rabbit pAb (A12401)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Fas Rabbit pAb (A12401)

Western blot analysis of extracts of various cell lines, using Fas antibody (A12401) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Fas Rabbit pAb (A12401)

Immunofluorescence analysis of A549 cells using Fas antibody (A12401). Blue: DAPI for nuclear staining.

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Overview

Product name Fas Rabbit pAb
Catalog No. A12401
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 30-335 of human Fas (NP_000034.1).
Sequence INSKGLELRKTVTTVETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLTSNTKCKEEGSRSNLGWLCLLLLPIPLIVWVKRKEVQKTCRKHRKENQGSHESPTLNPETVAINLSDVDLSKYITTIAGVMTLSQVKGFVRKNGVNEAKIDEIKNDNVQDTAEQKVQLLRNWHQLHGKKEAYDTLIKDLKKANLCTLAEKIQTIILKDITSDSENSNFRNEIQSLV
Gene ID 355
Swiss prot P25445
Synonyms APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6; Fas
Calculated MW 38kDa
Observed MW 40-50kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HT-1080, HepG2, HeLa
Cellular location Cell membrane, Secreted, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Lot number

Research Area

Fas Rabbit pAb images

ABclonal:Western blot - Fas Rabbit pAb (A12401)}

Western blot - Fas Rabbit pAb (A12401)

Western blot analysis of extracts of various cell lines, using Fas antibody (A12401) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - Fas Rabbit pAb (A12401)}

Immunofluorescence - Fas Rabbit pAb (A12401)

Immunofluorescence analysis of A549 cells using Fas antibody (A12401). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on FAS. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to FAS. (Distance between topics and target gene indicate popularity.) FAS

* Data provided by citexs.com, for reference only.

Publishing research using A12401? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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