Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ERRFI1 Rabbit pAb (A13099)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Rat

ABclonal:Western blot - ERRFI1 Rabbit pAb (A13099)

Western blot analysis of lysates from rat lung, using ERRFI1 Rabbit pAb (A13099) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5min.

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Overview

Product name ERRFI1 Rabbit pAb
Catalog No. A13099
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al., 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33, which is induced during cell stress and mediates cell signaling (Makkinje et al., 2000 [PubMed 10749885]; Fiorentino et al., 2000 [PubMed 11003669]).

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 223-462 of human ERRFI1 (NP_061821.1).
Sequence SYVSDQNGGVPDPNPPPPQTHRRLRRSHSGPAGSFNKPAIRISNCCIHRASPNSDEDKPEVPPRVPIPPRPVKPDYRRWSAEVTSSTYSDEDRPPKVPPREPLSPSNSRTPSPKSLPSYLNGVMPPTQSFAPDPKYVSSKALQRQNSEGSASKVPCILPIIENGKKVSSTHYYLLPERPPYLDKYEKFFREAEETNGGAQIQPLPADCGISSATEKPDSKTKMDLGGHVKRKHLSYVVSP
Gene ID 54206
Swiss prot Q9UJM3
Synonyms MIG6; RALT; MIG-6; GENE-33; ERRFI1
Calculated MW 51kDa
Observed MW 50kDa

Applications

Reactivity Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Rat lung
Cellular location Cell membrane, Cytoplasm, Cytoplasmic side, Nucleus, Peripheral membrane protein

Research Area

ERRFI1 Rabbit pAb images

ABclonal:Western blot - ERRFI1 Rabbit pAb (A13099)}

Western blot - ERRFI1 Rabbit pAb (A13099)

Western blot analysis of lysates from rat lung, using ERRFI1 Rabbit pAb (A13099) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5min.

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Related Targets

Based on existing publications, the following genes are closely related to research on ERRFI1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ERRFI1. (Distance between topics and target gene indicate popularity.) ERRFI1

* Data provided by citexs.com, for reference only.

Publishing research using A13099? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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