Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

ERK1/ERK2 Rabbit mAb (A11116)

Publications (5) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - ERK1/ERK2 Rabbit mAb (A11116)

Western blot analysis of extracts of various cell lines, using MAPK1/MAPK3 antibody (A11116).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name ERK1/ERK2 Rabbit mAb
Catalog No. A11116
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Immunogen information

Immunogen Recombinant protein of human MAPK3
Sequence Email for sequence
Gene ID 55945595
Swiss prot P28482P27361
Synonyms MAPK1/MAPK3; ERK1/ERK2
Calculated MW 36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW 41kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples HeLa, SW480, HCT116, PC-12
Cellular location caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane
Customer validation

WB (Homo sapiens, Mus musculus)

IF (Homo sapiens)

Research Area

ERK1/ERK2 Rabbit mAb images

ABclonal:Western blot - ERK1/ERK2 Rabbit mAb (A11116)}

Western blot - ERK1/ERK2 Rabbit mAb (A11116)

Western blot analysis of extracts of various cell lines, using MAPK1/MAPK3 antibody (A11116).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on MAPK1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAPK1. (Distance between topics and target gene indicate popularity.) MAPK1

* Data provided by citexs.com, for reference only.

Publishing research using A11116? Please let us know so that we can cite the reference in this datasheet.

Antibodies (8)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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