Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

EP300 Mouse mAb (A1324)

Review (1) Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - EP300 Mouse mAb (A1324)

Western blot analysis of extracts of Recombinant protein, using EP300 antibody (A1324).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Review
ABclonal: review for EP300 Mouse mAb(A1324)

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Overview

Product name EP300 Mouse mAb
Catalog No. A1324
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.

Immunogen information

Immunogen Recombinant Protein of human EP300
Sequence Email for sequence
Gene ID 2033
Swiss prot Q09472
Synonyms p300; KAT3B; MKHK2; RSTS2; EP300
Calculated MW 264kDa
Observed MW 17kDa/34kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Recombinant protein
Cellular location Cytoplasm, Nucleus
Customer validation

WB (SK-N-SH,SH-SY5Y,BE(2)CMus musculus)

Research Area

EP300 Mouse mAb images

ABclonal:Western blot - EP300 Mouse mAb (A1324)}

Western blot - EP300 Mouse mAb (A1324)

Western blot analysis of extracts of Recombinant protein, using EP300 antibody (A1324).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on EP300. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to EP300. (Distance between topics and target gene indicate popularity.) EP300

* Data provided by citexs.com, for reference only.

Publishing research using A1324? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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