Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

EED Rabbit pAb (A5371)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - EED Rabbit pAb (A5371)

Western blot analysis of various lysates, using EED Rabbit pAb (A5371) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - EED Rabbit pAb (A5371)

Immunofluorescence analysis of A-549 cells using EED Rabbit pAb (A5371) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EED Rabbit pAb (A5371)

Immunofluorescence analysis of C6 cells using EED Rabbit pAb (A5371) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EED Rabbit pAb (A5371)

Immunofluorescence analysis of NIH/3T3 cells using EED Rabbit pAb (A5371) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - EED Rabbit pAb (A5371)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg EED antibody (A5371). Western blot was performed from the immunoprecipitate using EED antibody (A5371) at a dilution of 1:1000.

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Overview

Product name EED Rabbit pAb
Catalog No. A5371
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-400 of human EED (NP_694536.1).
Sequence MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAK
Gene ID 8726
Swiss prot O75530
Synonyms HEED; COGIS; WAIT1; EED
Calculated MW 50kDa
Observed MW 50-70kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunoprecipitation    Immunofluorescence    
Positive samples HeLa, Mouse brain
Cellular location Chromosome, Nucleus
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

EED Rabbit pAb images

ABclonal:Western blot - EED Rabbit pAb (A5371)}

Western blot - EED Rabbit pAb (A5371)

Western blot analysis of various lysates, using EED Rabbit pAb (A5371) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - EED Rabbit pAb (A5371)}

Immunofluorescence - EED Rabbit pAb (A5371)

Immunofluorescence analysis of A-549 cells using EED Rabbit pAb (A5371) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - EED Rabbit pAb (A5371)}

Immunofluorescence - EED Rabbit pAb (A5371)

Immunofluorescence analysis of C6 cells using EED Rabbit pAb (A5371) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - EED Rabbit pAb (A5371)}

Immunofluorescence - EED Rabbit pAb (A5371)

Immunofluorescence analysis of NIH/3T3 cells using EED Rabbit pAb (A5371) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - EED Rabbit pAb (A5371)}

Immunoprecipitation - EED Rabbit pAb (A5371)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg EED antibody (A5371). Western blot was performed from the immunoprecipitate using EED antibody (A5371) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on EED. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to EED. (Distance between topics and target gene indicate popularity.) EED

* Data provided by citexs.com, for reference only.

Publishing research using A5371? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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