Product Type > Antibodies > Primary Antibodies > Methyl-specific Antibodies

MonoMethyl-UHRF1-K385 Rabbit pAb (A16008)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - MonoMethyl-UHRF1-K385 Rabbit pAb (A16008)

Western blot analysis of extracts of C6 cells, using MonoMethyl-UHRF1-K385 antibody (A16008) at 1:1000 dilution.C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Dot Blot - MonoMethyl-UHRF1-K385 Rabbit pAb (A16008)

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-UHRF1-K385 antibody (A16008) at 1:1000 dilution.

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Overview

Product name MonoMethyl-UHRF1-K385 Rabbit pAb
Catalog No. A16008
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.

Immunogen information

Immunogen A synthetic monomethylated peptide around K385 of human UHRF1 (NP_001041666.1).
Sequence KAKMA
Gene ID 29128
Swiss prot Q96T88
Synonyms Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95; MonoMethyl-UHRF1-K385
Calculated MW 90kDa
Observed MW 91kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • DB 1:500 - 1:2000
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples C6
Cellular location Nucleus

Documents

Certificate of Compliance

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MonoMethyl-UHRF1-K385 Rabbit pAb images

ABclonal:Western blot - MonoMethyl-UHRF1-K385 Rabbit pAb (A16008)}

Western blot - MonoMethyl-UHRF1-K385 Rabbit pAb (A16008)

Western blot analysis of extracts of C6 cells, using MonoMethyl-UHRF1-K385 antibody (A16008) at 1:1000 dilution.C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Dot Blot - MonoMethyl-UHRF1-K385 Rabbit pAb (A16008)}

Dot Blot - MonoMethyl-UHRF1-K385 Rabbit pAb (A16008)

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-UHRF1-K385 antibody (A16008) at 1:1000 dilution.

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Related Targets

Based on existing publications, the following genes are closely related to research on UHRF1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to UHRF1. (Distance between topics and target gene indicate popularity.) UHRF1

* Data provided by citexs.com, for reference only.

Publishing research using A16008? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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