Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Dot1L/KMT4 Rabbit pAb (A11286)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Dot1L/KMT4 Rabbit pAb (A11286)

Western blot analysis of extracts of various cell lines, using Dot1L/KMT4 antibody (A11286) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Overview

Product name Dot1L/KMT4 Rabbit pAb
Catalog No. A11286
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 250-500 of human Dot1L/KMT4 (NP_115871.1).
Sequence DHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRESKSNAATPTKGPEGKVAGPADAPMDSGAEEEKAGAATVKKPSPSKARKKKLNKKGRKMAGRKRGRPKKMNTANPERKPKKNQTALDALHAQTVSQTAASSPQDAYRSPHSPFYQLPPSVQRHSPNPLLVAPTPPALQKLLESFKIQYLQFLA
Gene ID 84444
Swiss prot Q8TEK3
Synonyms DOT1; KMT4; Dot1L/KMT4
Calculated MW 165kDa
Observed MW 170kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples BT-474, Mouse liver, Rat liver
Cellular location Nucleus

Research Area

Dot1L/KMT4 Rabbit pAb images

ABclonal:Western blot - Dot1L/KMT4 Rabbit pAb (A11286)}

Western blot - Dot1L/KMT4 Rabbit pAb (A11286)

Western blot analysis of extracts of various cell lines, using Dot1L/KMT4 antibody (A11286) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on DOT1L. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to DOT1L. (Distance between topics and target gene indicate popularity.) DOT1L

* Data provided by citexs.com, for reference only.

Publishing research using A11286? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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